Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970) - Azide and BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Golgi Glycoprotein 1/GLG1
Concentration
1.0 mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry, CyTOF
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Mouse (Negative), Rat (Negative)
Gene Alias
CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
Gene Symbols
GLG1
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Clone
GLG1/970
Dilution
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, Immunocytochemistry : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with No Preservative
Gene ID (Entrez)
2734
Immunogen
Golgi fraction from human liver cells
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
134 kDa
Related Products
Description
- Golgi Glycoprotein 1/GLG1 Monoclonal specifically detects Golgi Glycoprotein 1/GLG1 in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.
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Antigen: Golgi Glycoprotein 1/GLG1
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Mouse (Negative), Rat (Negative)
Gene Alias: CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
Gene Symbols: GLG1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Clone: GLG1/970
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, Immunocytochemistry : 0.5 - 1.0 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with No Preservative
Gene ID (Entrez): 2734
Immunogen: Golgi fraction from human liver cells
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 134 kDa
Antigen: Cytokeratin, LMW
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Rat
Gene Alias: Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
Gene Symbols: KRT77
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Clone: KRTL/1077
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with No Preservative
Gene ID (Entrez): 374454
Immunogen: Recombinant human KRT77 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Antigen: Cytokeratin, LMW
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Rat
Gene Alias: Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
Gene Symbols: KRT77
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Clone: KRTL/1077
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with No Preservative
Gene ID (Entrez): 374454
Immunogen: Recombinant human KRT77 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __