7105923

Elastase Antibody (CELA3B/1218) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Elastase

Concentration

1.0 mg/mL

Applications

Flow Cytometry, ELISA, Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Neuroscience

Formulation

PBS with No Preservative

Gene ID (Entrez)

23436

Immunogen

Recombinant human CELA3B protein

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone

CELA3B/1218

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Gene Symbols

CELA3B

Isotype

IgG κ

Purification Method

Protein A or G purified

Test Specificity

This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Description

  • Elastase Monoclonal specifically detects Elastase in Human samples
  • It is validated for Western Blot, ELISA, CyTOF-ready.

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Fischer Scientific

7105923

Mouse Monoclonal Antibody...


Antigen:
Elastase

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, ELISA, Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Neuroscience

Formulation:
PBS with No Preservative

Gene ID (Entrez):
23436

Immunogen:
Recombinant human CELA3B protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
CELA3B/1218

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Gene Symbols:
CELA3B

Isotype:
IgG κ

Purification Method:
Protein A or G purified

Test Specificity:
This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Img

Fischer Scientific

7105924

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 7

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
PBS with No Preservative

Gene ID (Entrez):
3855

Immunogen:
Recombinant full-length human KRT7 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
KRT7/760

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Img

Fischer Scientific

7105925

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 7

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
PBS with No Preservative

Gene ID (Entrez):
3855

Immunogen:
OTN 11, ovarian carcinoma cell line

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
OV-TL12/30

Dilution:
Simple Western : 1.0 ug/ml, Flow Cytometry : 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin : 1:100-1:200, Immunofluorescence : 1:50 - 1:100, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Img

Fischer Scientific

7105928

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 7

Concentration:
1.0 mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
PBS with No Preservative

Gene ID (Entrez):
3855

Immunogen:
Recombinant full-length human KRT7 protein (KRT7/760); OTN 11, ovarian carcinoma cell line (OV-TL12/30)

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone:
KRT7/760 + OV-TL12/30

Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).