TLR4 Antibody (76B357.1) - Azide Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 25 publications
Manufacturer: Fischer Scientific
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Antigen
TLR4
Concentration
1.0 mg/ml
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), KnockDown, Neutralization
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
Gene Accession No.
O00206
Gene ID (Entrez)
7099
Immunogen
This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
76B357.1
Dilution
Western Blot 1-3 ug/ml, Flow Cytometry 1ul/1 million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5ug/ml, Immunohistochemistry-Frozen 1:500, Flow (Cell Surface) reported in multiple pieces of scientific literature, Flow (Intracellular) reported in scientific literature (PMID 18577706), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize reported in scientific literature (PMID 26063617)
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Bovine, Mammalia
Gene Alias
ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene Symbols
TLR4
Isotype
IgG2b κ
Purification Method
Protein G purified
Test Specificity
The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
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Description
- TLR4 Monoclonal specifically detects TLR4 in Human, Mouse, Rat, Porcine, Bovine, Mammal samples
- It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Cell Surface), Flow (Intracellular), CyTOF-ready, Knockout Validated, Knockdown Validated, Block/Neutralize.
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Antigen: TLR4
Concentration: 1.0 mg/ml
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), KnockDown, Neutralization
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Toll Like Receptors
Gene Accession No.: O00206
Gene ID (Entrez): 7099
Immunogen: This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 76B357.1
Dilution: Western Blot 1-3 ug/ml, Flow Cytometry 1ul/1 million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5ug/ml, Immunohistochemistry-Frozen 1:500, Flow (Cell Surface) reported in multiple pieces of scientific literature, Flow (Intracellular) reported in scientific literature (PMID 18577706), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready, Knockout Validated, KnockDown Validated, Block/Neutralize reported in scientific literature (PMID 26063617)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Mammalia
Gene Alias: ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene Symbols: TLR4
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
Antigen: IL-6
Concentration: 0.5 mg/mL
Applications: __
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Adaptive Immunity, Biologically Active Proteins, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity, Neuroscience
Gene Accession No.: __
Gene ID (Entrez): 3569
Immunogen: COS-expressed human IL-6
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Clone: MQ2-13A5
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: __
Gene Alias: __
Gene Symbols: IL6
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Human/Rhesus/Cynomolgus IL-6
Antigen: IL-6
Concentration: 0.5 mg/mL
Applications: __
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Adaptive Immunity, Biologically Active Proteins, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity, Neuroscience
Gene Accession No.: __
Gene ID (Entrez): 3569
Immunogen: COS-expressed human IL-6
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Clone: MQ2-13A5
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: __
Gene Alias: __
Gene Symbols: IL6
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Human/Rhesus/Cynomolgus IL-6