7162871
RNA Polymerase II, Mouse anti-Human, Yeast, Clone: 8WG16, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
RNA Polymerase II
Dilution
Western Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Yeast
Gene Alias
DNA-directed RNA polymerase II subunit RPB1;EC: 2.7.7.6;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Gene Symbols
POLR2A;POLR2
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
Clone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
Clone
8WG16
Applications
ChIP Assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene ID (Entrez)
NP_000928
Immunogen
RNA Polymerase II from wheat germ extract
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen
∽220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Description
- Anti-RNA Polymerase II, CTD, clone 8WG16, Cat
- No
- 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting
- DNA-directed RNA polymerase II subunit RPB1 (UniProt: P24928; also known as EC: 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, is encoded by the POLR2A (also known as POLR2) gene (Gene ID: 5430) in human
- DNA-dependent RNA polymerase II catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
- RPB1 subunit is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that suggested to grab the incoming DNA template
- It has multiple zinc and magnesium ion binding sites and contains a repetitive C-terminal domain (CTD; aa 1593-1960) that consists of tandem repeats of the consensus sequence YSPTSPS and serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing
- It can exist in both hypophosphorylated form, which is mainly cytosolic, and in hyperphosphorylated form
- The phosphorylation of Serine 2 and 5 in the hepatapeptide repeat is achieved by Cdk7 and Cdk9
- Cdk7 phosphorylation promotes transcription initiation by triggering promoter release and elongation.
Compare Similar Items
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Antigen: RNA Polymerase II
Dilution: Western Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Yeast
Gene Alias: DNA-directed RNA polymerase II subunit RPB1;EC: 2.7.7.6;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Gene Symbols: POLR2A;POLR2
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
Clone: 8WG16
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene ID (Entrez): NP_000928
Immunogen: RNA Polymerase II from wheat germ extract
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
RNA Polymerase II
Dilution:
Western Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Yeast
Gene Alias:
DNA-directed RNA polymerase II subunit RPB1;EC: 2.7.7.6;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Gene Symbols:
POLR2A;POLR2
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
Clone:
8WG16
Applications:
ChIP Assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene ID (Entrez):
NP_000928
Immunogen:
RNA Polymerase II from wheat germ extract
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: Neurogranin
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Rat, Human
Gene Alias: Ng;RC3
Gene Symbols: NRGN
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Clone: __
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001119653
Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
Neurogranin
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Rat, Human
Gene Alias:
Ng;RC3
Gene Symbols:
NRGN
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Clone:
__
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001119653
Immunogen:
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: Vesicular Glutamate Transporter 3 (VGLUT3)
Dilution: Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Mouse, Human, Rat
Gene Alias: Solute carrier family 17 member 8;VGluT3
Gene Symbols: Slc17a8;Vglut3
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Clone: __
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
Vesicular Glutamate Transporter 3 (VGLUT3)
Dilution:
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Mouse, Human, Rat
Gene Alias:
Solute carrier family 17 member 8;VGluT3
Gene Symbols:
Slc17a8;Vglut3
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Clone:
__
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: PDGF-C
Dilution: Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Gene Symbols: PDGFC;SCDGE;UNQ174/PRO200
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Clone: __
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_057289
Immunogen: A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
PDGF-C
Dilution:
Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Gene Symbols:
PDGFC;SCDGE;UNQ174/PRO200
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Clone:
__
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_057289
Immunogen:
A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).