7162872
Neurogranin, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Neurogranin
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Rat, Human
Gene Alias
Ng;RC3
Gene Symbols
NRGN
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001119653
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Related Products
Description
- Anti-Neurogranin Antibody, Cat
- No
- 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human
- Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum
- In neurons, it is found concentrated at dendritic spines
- It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling
- It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78)
- The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA)
- Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation
- Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions
- Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease
- (Ref.: Javier Dı´ez-Guerra, F
- (2010)
- Life
- 62(8): 597-606; Casaletto, KB et al
- (2017)
- Neurology 89(17); 1782-1788).
Compare Similar Items
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Antigen: Neurogranin
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Rat, Human
Gene Alias: Ng;RC3
Gene Symbols: NRGN
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001119653
Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
Neurogranin
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Rat, Human
Gene Alias:
Ng;RC3
Gene Symbols:
NRGN
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001119653
Immunogen:
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: Vesicular Glutamate Transporter 3 (VGLUT3)
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Mouse, Human, Rat
Gene Alias: Solute carrier family 17 member 8;VGluT3
Gene Symbols: Slc17a8;Vglut3
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Dilution: Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
Vesicular Glutamate Transporter 3 (VGLUT3)
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Mouse, Human, Rat
Gene Alias:
Solute carrier family 17 member 8;VGluT3
Gene Symbols:
Slc17a8;Vglut3
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Dilution:
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: PDGF-C
Applications: Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human
Gene Alias: Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Gene Symbols: PDGFC;SCDGE;UNQ174/PRO200
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Dilution: Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_057289
Immunogen: A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
PDGF-C
Applications:
Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Gene Symbols:
PDGFC;SCDGE;UNQ174/PRO200
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Dilution:
Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_057289
Immunogen:
A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: KLF4
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Gene Symbols: Klf4;Ezf;Gklf;Zie
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Epigenetics & Nuclear Function
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez): NP_034767
Immunogen: GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
KLF4
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Gene Symbols:
Klf4;Ezf;Gklf;Zie
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez):
NP_034767
Immunogen:
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).