7162879
KLF4, Rabbit anti-Mouse, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
KLF4
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Epigenetics & Nuclear Function
Formulation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez)
NP_034767
Immunogen
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification
Polyclonal
Form
Serum
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Gene Symbols
Klf4;Ezf;Gklf;Zie
Isotype
IgG
Purification Method
Unpurified
Test Specificity
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Description
- Anti-KLF4, Cat
- No
- ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting
- Krueppel-like factor 4 (UniProt: Q60793; also known as Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4) is encoded by the Klf4 (also known as Ezf, Gklf, Zie) gene (Gene ID: 16600) in murine species
- Klf4 is a member of the krueppel C2H2-type zinc-finger protein family that regulates multiple biological processes, including proliferation, differentiation, development, and apoptosis
- KLF4 is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and can be used as a marker of the pluripotent state for cells in culture
- It can serve both as activator and as repressor depending upon its environment, but its most prominent function is growth suppression
- Interestingly, increased expression of Klf4 leads to an increase in expression of P21WAF1/CIP1, which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries
- Klf4 also mediates the normal functions of the tumor suppressor p53 and contributes to the down-regulation of p53/TP53 transcription
- Mutations in Klf4 gene can lead to unchecked growth and tumor formation
- KLF4 is shown to be often mutated in many colon cancer cell lines and the lack of Klf4 expression is correlated with colorectal cancer pathogenesis
- Elevated expression of Klf4 gene has been linked to gastric cancer, breast cancer, and squamous cell carcinomas
- KLF4 is reported to target the primary miR1-2 loop promoter and stimulates miR-1 expression
- Loss of KLF4 expression is considered as a mechanistic link between aggressive prostate cancer progression and low canonical AR output through miR-1 inactivation
- (Ref.: Jiang, J., et al
- (2008)
- Nat
- Cell Biol
- 10(3); 353-360; Siu, MK et al
- (2016)
- Oncogenesis 5: e282).
Compare Similar Items
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Antigen: KLF4
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Epigenetics & Nuclear Function
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez): NP_034767
Immunogen: GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification: Polyclonal
Form: Serum
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Gene Symbols: Klf4;Ezf;Gklf;Zie
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Antigen:
KLF4
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez):
NP_034767
Immunogen:
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification:
Polyclonal
Form:
Serum
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Gene Symbols:
Klf4;Ezf;Gklf;Zie
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Antigen: Tau (TauC4
Applications: Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez): NP_005901
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 45.85 kDa calculated.
Dilution: Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification: Polyclonal
Form: Serum
Regulatory Status: RUO
Target Species: Human
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen:
Tau (TauC4
Applications:
Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez):
NP_005901
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 45.85 kDa calculated.
Dilution:
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification:
Polyclonal
Form:
Serum
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen: Tau (TauC4
Applications: Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez): NP_005901
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 45.85 kDa calculated.
Dilution: Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification: Polyclonal
Form: Serum
Regulatory Status: RUO
Target Species: Human
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen:
Tau (TauC4
Applications:
Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez):
NP_005901
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 45.85 kDa calculated.
Dilution:
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification:
Polyclonal
Form:
Serum
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen: phospho AEP (Ser226)
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_005597
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols: LGMN;PRSC1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen:
phospho AEP (Ser226)
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_005597
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols:
LGMN;PRSC1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.