7162883
Tau (TauC4, Rabbit anti-Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Tau (TauC4
Applications
Immunomicroscopy, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez)
NP_005901
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen
∽55 kDa observed; 45.85 kDa calculated.
Dilution
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification
Polyclonal
Form
Serum
Regulatory Status
RUO
Target Species
Human
Gene Alias
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols
MAPT;MAPTL;MTBT1;TAU
Isotype
IgG
Purification Method
Unpurified
Test Specificity
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Description
- Anti-Tau (TauC4, 354-369), Cat
- No
- ABN2178, is a highly specific rabbit polyclonal antibody that targets TauC4 region of Tau and has been tested for use in Electron Microscopy and Western Blotting
- Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human
- Microtubule-associated protein tau is shown to be expressed in neurons
- It is mostly found in axons, in the cytosol, and in association with plasma membrane components
- Nine isoforms of Tau have been described that are generated by alternative splicing
- Tau proteins promote microtubule assembly and stability and may also be involved in the establishment and maintenance of neuronal polarity
- The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, thus serving as a linker protein between both
- The short isoforms are reported to allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization
- In Alzheimer disease (AD), the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of Tau
- In AD brain Tau accumulates in both the somatodendritic and axonal domains of neurons and it also accumulates in the soma as neurofibrillary tangles (NFTs)
- Tau oligomers are reported to be neurotoxic when applied extracellularly to cultured neuronal cells and can induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo
- It has been reported that most of the C-terminus of Tau (aa 243-406) in PHFs can be cleaved by trypsin, however the TauC4 region is highly resistant to trypsin action
- (Ref.: Taniguchi Watanabe, S., et al
- (2016)
- Acta Neuropathol
- 131(2); 267-280; Ando K., et al
- (2010)
- Biochem
- Soc
- Trans
- 38(4); 1001-1005).
Compare Similar Items
Show Difference
Antigen: Tau (TauC4
Applications: Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez): NP_005901
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 45.85 kDa calculated.
Dilution: Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification: Polyclonal
Form: Serum
Regulatory Status: RUO
Target Species: Human
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen:
Tau (TauC4
Applications:
Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene ID (Entrez):
NP_005901
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 45.85 kDa calculated.
Dilution:
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification:
Polyclonal
Form:
Serum
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Antigen: phospho AEP (Ser226)
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_005597
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols: LGMN;PRSC1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen:
phospho AEP (Ser226)
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_005597
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols:
LGMN;PRSC1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen: phospho AEP (Ser226)
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez): NP_005597
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols: LGMN;PRSC1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen:
phospho AEP (Ser226)
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez):
NP_005597
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 226 of human Asparaginyl endopeptidase (AEP).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽73 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected phospho AEP (Ser226) in lysates from HEK293 cells co-transfected mammalian GST-tagged (mGST) AEP and wild-type (WT) SRPK2. (Courtesy of Keqiang Ye, Ph.D., Professor, Emory University School of Medicine, Department of Pathology and Laboratory Medicine).Immunofluorescence Analysis: A representative lot detected phospho AEP (Ser226) in Immunofluorescence applications (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).Kinase Assay: A representative lot detected phospho AEP (Ser226) in HEK293 cells treated with SRPK2 and in vitro kinase assays (Wang, Z.H., et. al. (2017). Mol Cell. 67(5):812-825).
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Legumain;Asparaginyl endopeptidase;Protease cysteine 1;Delta Secretase
Gene Symbols:
LGMN;PRSC1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Asparaginyl endopeptidase phosphorylated on Serine 226.
Antigen: Aquaporin 4
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
Gene ID (Entrez): NP_001257487.1
Immunogen: GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution: Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Target Species: Rat, Mouse, Human
Gene Alias: AQP-4;Mercurial-insensitive water channel;MIWC
Gene Symbols: Aqp4
Isotype: __
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.
Antigen:
Aquaporin 4
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.
Gene ID (Entrez):
NP_001257487.1
Immunogen:
GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution:
Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Target Species:
Rat, Mouse, Human
Gene Alias:
AQP-4;Mercurial-insensitive water channel;MIWC
Gene Symbols:
Aqp4
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.