7162873
Vesicular Glutamate Transporter 3 (VGLUT3), Rabbit anti-Mouse, Human, Rat, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Vesicular Glutamate Transporter 3 (VGLUT3)
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Mouse, Human, Rat
Gene Alias
Solute carrier family 17 member 8;VGluT3
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Dilution
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Slc17a8;Vglut3
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Related Products
Description
- Anti-Vesicular Glutamate Transporter 3 (VGLUT3), Cat
- No
- AB5421-II, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 3 and has been tested foe use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting
- Vesicular glutamate transporter 3 (UniProt Q7TSF2; also known as Solute carrier family 17 member 8, VGluT3) is encoded by the Slc17a8 (also known as Vglut3) gene (Gene ID 266767) in rat species
- VGluTs are proton-dependent carriers that are responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft
- VGluT3 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons
- Rat VGluT3 has 12 helical, 7 cytoplasmic, and 6 vesicular domains
- It is structurally similar to VGluT1 and -2, but differs in its distribution pattern
- Unlike VGluT1 and 2 that rely on interactions with the endocytic machinery for recycling, but remain almost exclusively axonal, VGluT3 can target to the cell body and dendrites, as well as the axon of certain neurons and is implicated in the retrograde synaptic release of glutamate
- VGluT3 displays a very restricted localization and is not present in known glutamatergic neurons, but is found in cholinergic neurons in the caudate-putamen and in serotonergic neurons in the raphe nucleus
- VGluT3 is localized over vesicle clusters in both symmetric (inhibitory or modulatory) and asymmetric (excitatory) synapses
- (Ref.: Herzog, E et al
- (2004)
- Neuroscience
- 123(4):983-1002; Seal, RP et al
- (2008)
- Neuron 57(2); 263-275).
Compare Similar Items
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Antigen: Vesicular Glutamate Transporter 3 (VGLUT3)
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Mouse, Human, Rat
Gene Alias: Solute carrier family 17 member 8;VGluT3
Immunogen: KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Dilution: Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Slc17a8;Vglut3
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
Vesicular Glutamate Transporter 3 (VGLUT3)
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Mouse, Human, Rat
Gene Alias:
Solute carrier family 17 member 8;VGluT3
Immunogen:
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
Dilution:
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Slc17a8;Vglut3
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: PDGF-C
Applications: Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human
Gene Alias: Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Immunogen: A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Purification Method: Affinity Purified
Test Specificity: This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Dilution: Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene Symbols: PDGFC;SCDGE;UNQ174/PRO200
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen: ∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
PDGF-C
Applications:
Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Immunogen:
A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Purification Method:
Affinity Purified
Test Specificity:
This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Dilution:
Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene Symbols:
PDGFC;SCDGE;UNQ174/PRO200
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: KLF4
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Immunogen: GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Epigenetics & Nuclear Function
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Symbols: Klf4;Ezf;Gklf;Zie
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen:
KLF4
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Immunogen:
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Symbols:
Klf4;Ezf;Gklf;Zie
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Antigen: Tau (TauC4
Applications: Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Dilution: Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Neuroscience
Formulation: Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen: ∽55 kDa observed; 45.85 kDa calculated.
Antigen:
Tau (TauC4
Applications:
Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Immunogen:
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Microtubule-associated protein tau, isoform 8 (Tau-F).
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody specifically detects TauC4 region in human Tau protein.
Dilution:
Western Blotting Analysis: A representative lot detected Tau (TauC4, 354-369) in Western Blotting applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190; Taniguchi-Watanabe, S., et. al. (2016). Acta Neuropathol. 131(2):267-280).Electron Microscopy Analysis: A representative lot detected Tau (TauC4, 354-369) in Immunogold negative-stain Electron Microscopy applications (Fitzpatrick, A.W.P., et. al. (2017) Nature. 547(7662):185-190).
Classification:
Polyclonal
Host Species:
Rabbit
Research Discipline:
Neuroscience
Formulation:
Rabbit polyclonal antiserum with 0.1% sodium azide.
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
∽55 kDa observed; 45.85 kDa calculated.