7162899
GFP, Mouse anti-All, Clone: 2B6, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
GFP
Dilution
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
All species
Gene Alias
Green fluorescent protein
Immunogen
His-tagged recombinant jellyfish Green fluorescent protein.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Clone
2B6
Applications
Immunocytochemistry, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Secondary & Control Antibodies
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
GFP
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
Related Products
Description
- Anti-GFP, clone 2B6, Cat
- No
- MABC1689, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has been tested for use in Immunocytochemistry and Western Blotting
- Green fluorescent protein (UniProt: P42212) is encoded by the GFP gene in Aequorea victoria (Jellyfish)
- Green fluorescence protein (GFP) is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor
- It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin
- GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm
- However, the exact value of emission max depends on the environment of the chromophore
- GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions.
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Antigen: GFP
Dilution: Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Green fluorescent protein
Immunogen: His-tagged recombinant jellyfish Green fluorescent protein.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 2B6
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Secondary & Control Antibodies
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: GFP
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
Antigen:
GFP
Dilution:
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Green fluorescent protein
Immunogen:
His-tagged recombinant jellyfish Green fluorescent protein.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
2B6
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Secondary & Control Antibodies
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
GFP
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
Antigen: Retinal Dehydrogenase 1/ALDH1A1
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Retinal Dehydrogenase 1/ALDH1A1 in human liver and rat liver tissue sections.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat
Gene Alias: Retinal dehydrogenase 1;EC: 1.2.1.36;RALDH 1;RalDH1;ALDH-E1;ALHDII;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase;cytosolic
Immunogen: KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human retinal dehydrogenase 1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 16B4.2
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Neuroscience
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ALDH1A;ALDC;ALDH1;PUMB1
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 16B4.2 detects Retinal dehydrogenase 1/ALDH1A1. it targets an epitope with in 18 ammo acids from the C-terminal region.
Antigen:
Retinal Dehydrogenase 1/ALDH1A1
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Retinal Dehydrogenase 1/ALDH1A1 in human liver and rat liver tissue sections.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Rat
Gene Alias:
Retinal dehydrogenase 1;EC: 1.2.1.36;RALDH 1;RalDH1;ALDH-E1;ALHDII;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase;cytosolic
Immunogen:
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human retinal dehydrogenase 1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
16B4.2
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Neuroscience
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ALDH1A;ALDC;ALDH1;PUMB1
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 16B4.2 detects Retinal dehydrogenase 1/ALDH1A1. it targets an epitope with in 18 ammo acids from the C-terminal region.
Antigen: ESET (SETDB1)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Immunogen: His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: F0808
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols: Setdb1;Eset;Kiaa0067
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Antigen:
ESET (SETDB1)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Immunogen:
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
F0808
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols:
Setdb1;Eset;Kiaa0067
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Antigen: ESET (SETDB1)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Immunogen: His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: F0808
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols: Setdb1;Eset;Kiaa0067
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Antigen:
ESET (SETDB1)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Immunogen:
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
F0808
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols:
Setdb1;Eset;Kiaa0067
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.