7162889
FtsZ (GTPase), Rabbit anti-Bacteria, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
FtsZ (GTPase)
Applications
Immunofluorescence, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Signaling
Formulation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez)
NP_389412
Immunogen
Full length purified FtsZ from Bacillus subtilis.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Dilution
Western Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).
Classification
Polyclonal
Form
Serum
Regulatory Status
RUO
Target Species
Bacteria
Gene Alias
Cell division protein FtsZ;Cell Division FtsZ GTPase
Gene Symbols
FtsZ;BSU15290
Isotype
IgG
Purification Method
Unpurified
Test Specificity
This rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.
Description
- Anti-FtsZ (GTPase), Cat
- No
- ABS2200, is a rabbit polyclonal antibody that detects Cell division protein FtsZ and has been tested for use in Immunofluorescence and Western Blotting
- Cell division protein FtsZ (UniProt: P17865; also known as Cell Division FtsZ GTPase, FtsZ) is encoded by the FtsZ (also known as BSU15290) gene (Gene ID: 935971) in Bacillus subtilis
- FtsZ is homodimeric prokaryotic homologue of tubulin with GTPase activity
- It is found in nearly all bacteria
- It polymerizes in the presence of GTP to form higher-ordered polymers
- It contains three GTP-binding sites that are located in the N-terminal region
- The less conserved C-terminal domain contains residues that are important for GTP hydrolysis
- FtsZ is shown to be essential for bacterial cell division and in association with FtzA (FtsAZ) it forms a contractile ring structure (Z ring) at the site of cell division
- The regulation of the ring assembly controls the timing and the location of cell division
- One of the major functions of FtsZ is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells
- The FtsZ treadmilling rate controls the rate of peptidoglycan synthesis and cell division
- During bacterial cell division FtsZ co-localizes with SpoIIE, a dual function protein that contributes to the switch form medial to polar cell division during sporulation
- The anti-FtsZ blot has also been used as control for sample loading
- (Ref.: Bisson-Filho, AW., et al., (2017)
- Science 355(6326); 739-743; Feucht, A., and Jeffery Errington, J (2005)
- Microbiology 151(6); 2053-2064; Lucet, I., et al
- (2000)
- EMBO J
- 19(7); 1467-1475)
- Lytvynenko, I., et al
- (2019)
- Cell
- 178(1); 76-90)
Compare Similar Items
Show Difference
Antigen: FtsZ (GTPase)
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Signaling
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez): NP_389412
Immunogen: Full length purified FtsZ from Bacillus subtilis.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Dilution: Western Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).
Classification: Polyclonal
Form: Serum
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: Cell division protein FtsZ;Cell Division FtsZ GTPase
Gene Symbols: FtsZ;BSU15290
Isotype: IgG
Purification Method: Unpurified
Test Specificity: This rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.
Antigen:
FtsZ (GTPase)
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Signaling
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene ID (Entrez):
NP_389412
Immunogen:
Full length purified FtsZ from Bacillus subtilis.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Dilution:
Western Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).
Classification:
Polyclonal
Form:
Serum
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
Cell division protein FtsZ;Cell Division FtsZ GTPase
Gene Symbols:
FtsZ;BSU15290
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
This rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.
Antigen: CD34
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001020280
Immunogen: Human placental endothelial membrane vesicles.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Monkey, Human
Gene Alias: Hematopoietic progenitor cell antigen CD34
Gene Symbols: CD34
Isotype: IgG1 λ
Purification Method: Protein G purified
Test Specificity: Clone QBEnd/10 specifically detects CD34 in human and non-human primates.
Antigen:
CD34
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001020280
Immunogen:
Human placental endothelial membrane vesicles.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Monkey, Human
Gene Alias:
Hematopoietic progenitor cell antigen CD34
Gene Symbols:
CD34
Isotype:
IgG1 λ
Purification Method:
Protein G purified
Test Specificity:
Clone QBEnd/10 specifically detects CD34 in human and non-human primates.
Antigen: BASE-tag
Applications: __
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Virus
Gene Alias: Capsid protein VP1;Capsid protein AVV9
Gene Symbols: Cap
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Antigen:
BASE-tag
Applications:
__
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus
Gene Alias:
Capsid protein VP1;Capsid protein AVV9
Gene Symbols:
Cap
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Antigen: BASE-tag
Applications: __
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Virus
Gene Alias: Capsid protein VP1;Capsid protein AVV9
Gene Symbols: Cap
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Antigen:
BASE-tag
Applications:
__
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus
Gene Alias:
Capsid protein VP1;Capsid protein AVV9
Gene Symbols:
Cap
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.