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7162890

CD34, Mouse anti-Monkey, Human, Clone: QBEnd/10, Millipore Sigma™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD34

Dilution

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood.

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Monkey, Human

Gene Alias

Hematopoietic progenitor cell antigen CD34

Gene Symbols

CD34

Isotype

IgG1 λ

Purification Method

Protein G purified

Test Specificity

Clone QBEnd/10 specifically detects CD34 in human and non-human primates.

Clone

QBEnd/10

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Inflammation & Immunology

Formulation

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_001020280

Immunogen

Human placental endothelial membrane vesicles.

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Molecular Weight of Antigen

40.72 kDa Calculated. This antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa.

Description

  • Anti-CD34, clone QBEnd/10, Cat
  • No
  • CBL496-I, is a mouse monoclonal antibody that detects CD34 and has been tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence and Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (Paraffin), and Western Blotting
  • Hematopoietic progenitor cell antigen CD34 (UniProt: P28906; also known as CD34) is encoded by the CD34 gene (Gene ID: 947) in human
  • CD34 is a highly glycosylated single-pass type I membrane protein that is expressed on hematopoietic progenitor cells and small vessel endothelium of a variety of tissues
  • Under normal conditions, CD34+ expressing cells account for about 1 2% of the total bone marrow cells
  • It serves as an adhesion molecule that plays a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells
  • It is also reported to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components
  • CD34 is synthesized with a signal peptide (aa 1-31) that is cleaved off in the mature form
  • The mature form has an extracellular domain (aa 32-290), a transmembrane domain (aa 291-311), and a cytoplasmic domain (aa 312-385)
  • Two isoforms of CD34 have been described that are produced by alternative splicing.

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7162890

Mouse Monoclonal Antibody...

Antigen:
CD34
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Monkey, Human
Gene Alias:
Hematopoietic progenitor cell antigen CD34
Gene Symbols:
CD34
Isotype:
IgG1 λ
Purification Method:
Protein G purified
Test Specificity:
Clone QBEnd/10 specifically detects CD34 in human and non-human primates.
Clone:
QBEnd/10
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001020280
Immunogen:
Human placental endothelial membrane vesicles.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Molecular Weight of Antigen:
40.72 kDa Calculated. This antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa.

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Antigen:
BASE-tag
Dilution:
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Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus
Gene Alias:
Capsid protein VP1;Capsid protein AVV9
Gene Symbols:
Cap
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Clone:
7C7
Applications:
__
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
__

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Mouse Monoclonal Antibody...

Antigen:
BASE-tag
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus
Gene Alias:
Capsid protein VP1;Capsid protein AVV9
Gene Symbols:
Cap
Isotype:
IgG2b κ
Purification Method:
Protein G purified
Test Specificity:
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
Clone:
7C7
Applications:
__
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
Empty Adeno-associated virus 9 (AAV9) Capsids
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
__

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Mouse Monoclonal Antibody...

Antigen:
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Dilution:
Immunofluorescence Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Immunofluorescence applications (Barton, E.S., et. al. (2001). J Biol Chem. 276(3):2200-2211).Radioimmunoassay Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Radioimmunoassay applications (William, W.V., et. al. (1988). Proc Natl Acad Sci U S A. 85(17):6488-92). Flow Cytometry Analysis: A representative lot detected Reovirus Type 3 sigma 1 hemagglutinin in Flow Cytometry applications (Dietrich, M.H., et. al. (2017). J Virol. 91(4). pii: e01621-16).Neutralizing Analysis: A representative lot neutralized Reovirus Type 3 in Neutralizing applications (William, W.V., et. al. (1988). Proc Natl Acad Sci USA. 85(17):6488-92).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Virus
Gene Alias:
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Gene Symbols:
N/A
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
__
Clone:
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Applications:
Flow Cytometry, Immunofluorescence, Neutralization, Radioimmune Assays (RIA)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
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Gene ID (Entrez):
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Immunogen:
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Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen:
N/A