7162896
PD-L1 B7-H1, Armenian Hamster anti-Mouse, Clone: 10B5, Millipore Sigma™
Armenian Hamster Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PD-L1 B7-H1
Dilution
Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722). Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Programmed cell death 1 ligand 1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274
Gene Symbols
Cd274;B7h1;Pdcd1l1;Pdcd1lg1;Pdl1
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone
10B5
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition Assays
Conjugate
Unconjugated
Host Species
Armenian Hamster
Research Discipline
Apoptosis & Cancer
Formulation
Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Gene ID (Entrez)
NP_068693.1
Immunogen
Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.
Purification Method
Protein G purified
Test Specificity
This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.
Description
- Anti-PD-L1 B7-H1, clone 10B5, Cat
- No
- MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay
- Programmed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the Cd274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 50533) in murine species
- PD-L1 is a single-pass type I membrane protein that is highly expressed in the heart, thymus, skeletal muscle, and lung
- Its expression is also reported on activated dendritic cells, B-cells, and macrophages
- Numerous tumor cell lines of lymphoid origin also express PD-L1
- PD-L1 expression can be up-regulated by interferon-gamma treatment in monocytes, B cells, and macrophages
- PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained
- However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity
- The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells
- PD-L1 is initially produced with signal peptide (aa
- 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa
- 19-239) domain, a transmembrane domain (aa 240-260), and a cytoplasmic domain (aa 261-290).
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Antigen: PD-L1 B7-H1
Dilution: Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722). Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Programmed cell death 1 ligand 1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274
Gene Symbols: Cd274;B7h1;Pdcd1l1;Pdcd1lg1;Pdl1
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone: 10B5
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition Assays
Conjugate: Unconjugated
Host Species: Armenian Hamster
Research Discipline: Apoptosis & Cancer
Formulation: Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Gene ID (Entrez): NP_068693.1
Immunogen: Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.
Purification Method: Protein G purified
Test Specificity: This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.
Antigen:
PD-L1 B7-H1
Dilution:
Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722). Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Programmed cell death 1 ligand 1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274
Gene Symbols:
Cd274;B7h1;Pdcd1l1;Pdcd1lg1;Pdl1
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clone:
10B5
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition Assays
Conjugate:
Unconjugated
Host Species:
Armenian Hamster
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Gene ID (Entrez):
NP_068693.1
Immunogen:
Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.
Purification Method:
Protein G purified
Test Specificity:
This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.
Antigen: ALK
Dilution: Western Blotting Analysis: A representative lot detected ALK in Western Blotting applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunofluorescence Analysis: A representative lot detected ALK in Immunofluorescence applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunoprecipitation Analysis: A representative lot immunoprecipitated ALK in Immunoprecipitation applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry (Paraffin) Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: ALK tyrosine kinase receptor;EC: 2.7.10.1;Anaplastic lymphoma kinase;CD246
Gene Symbols: ALK
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: ALK1
Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_004295
Immunogen: His-tagged full length human recombinant ALK tyrosine kinase receptor.
Purification Method: Protein G purified
Test Specificity: Clone ALK1 specifically detects human ALK tyrosine kinase receptor. It targets an epitope with in 102 amino acids from the C-terminal region.
Antigen:
ALK
Dilution:
Western Blotting Analysis: A representative lot detected ALK in Western Blotting applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunofluorescence Analysis: A representative lot detected ALK in Immunofluorescence applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunoprecipitation Analysis: A representative lot immunoprecipitated ALK in Immunoprecipitation applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).Immunohistochemistry (Paraffin) Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
ALK tyrosine kinase receptor;EC: 2.7.10.1;Anaplastic lymphoma kinase;CD246
Gene Symbols:
ALK
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
ALK1
Applications:
Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_004295
Immunogen:
His-tagged full length human recombinant ALK tyrosine kinase receptor.
Purification Method:
Protein G purified
Test Specificity:
Clone ALK1 specifically detects human ALK tyrosine kinase receptor. It targets an epitope with in 102 amino acids from the C-terminal region.
Antigen: Tex19.1
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections.Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Testis-expressed protein 19.1;mTex19.1;Testis-expressed protein 19A
Gene Symbols: Tex19.1;Tex19;Tex19a
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 7TEX-1F11
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_082878
Immunogen: His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19).
Purification Method: Protein G purified
Test Specificity: Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19).
Antigen:
Tex19.1
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections.Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Testis-expressed protein 19.1;mTex19.1;Testis-expressed protein 19A
Gene Symbols:
Tex19.1;Tex19;Tex19a
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
7TEX-1F11
Applications:
ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_082878
Immunogen:
His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19).
Purification Method:
Protein G purified
Test Specificity:
Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19).
Antigen: GFP
Dilution: Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Green fluorescent protein
Gene Symbols: GFP
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 2B6
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Secondary & Control Antibodies
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): __
Immunogen: His-tagged recombinant jellyfish Green fluorescent protein.
Purification Method: Protein G purified
Test Specificity: Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
Antigen:
GFP
Dilution:
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Green fluorescent protein
Gene Symbols:
GFP
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
2B6
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Secondary & Control Antibodies
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
__
Immunogen:
His-tagged recombinant jellyfish Green fluorescent protein.
Purification Method:
Protein G purified
Test Specificity:
Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.