7162898

Tex19.1, Mouse anti-Mouse, Clone: 7TEX-1F11, Millipore Sigma™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Tex19.1

Dilution

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections.Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Alias

Testis-expressed protein 19.1;mTex19.1;Testis-expressed protein 19A

Gene Symbols

Tex19.1;Tex19;Tex19a

Isotype

IgG2b κ

Purification Method

Protein G purified

Test Specificity

Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19).

Clone

7TEX-1F11

Applications

ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Signaling

Formulation

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez)

NP_082878

Immunogen

His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19).

Primary or Secondary

Primary

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

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Description

  • Anti-Tex19.1, clone 7TEX-1F11, Cat
  • No
  • MABC1596, is a mouse monoclonal antibody that detects Testis-expressed protein 19.1 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting
  • Testis-expressed protein 19.1 (UniProt: Q99MV2; also known as mTex19.1, Testis-expressed protein 19A) is encoded by the Tex19.1 (also known as Tex19, Tex19a) gene (Gene ID: 73679) in murine species
  • Tex19.1 resides in the germline to repress transposable genetic elements and maintain genomic stability
  • It is expressed in testis, placenta, ovary, and in pluripotent stem cells
  • In testis its expression is highest in mitotic spermatogonia and decreases as spermatocytes progress through meiosis
  • Tex19.1 expression is reported to be down-regulated by Deleted in azoospermia-like (DAZL) protein, which binds to 3'UTR of Tex19.1 mRNAs and repress its translation
  • Tex19.1 is required during spermatogenesis and placenta development where it participates in the repression of retrotransposable elements and prevents their mobilization
  • Tex19.1, along with its paralog, Tex19.2, is shown to collaborate with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins
  • Embryos with mutations in Tex19.1 gene exhibit intra-uterine growth retardation and have small placentas due to a reduction in the number of spongiotrophoblast, glycogen trophoblast and sinusoidal trophoblast giant cells
  • Loss of Tex19.1 is shown to cause growth defect and early post-natal lethality
  • Male animals with deficiency of both Tex19.1 and Tex19.2 display impaired spermatogenesis, small testes, and are infertility.

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7162898

Mouse Monoclonal Antibody...


Antigen:
Tex19.1

Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Tex19.1 in embryonic mouse brain and embryonic mouse gut tissue sections.Immunocytochemistry Analysis: A representative lot detected Tex19.1 in Immunocytochemistry applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).ELISA Analysis: A representative lot detected Tex19.1 in ELISA applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14).Immunoprecipitation Analysis: A representative lot immunoprecipitated Tex19.1 in Immunoprecipitation applications (Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).Western Blotting Analysis: A representative lot detected Tex19.1 in Western Blotting applications (Tarabay, Y., et. al. (2013). Hum Reprod. 28(8):2201-14;Tarabay, Y., et. al. (2017). J Cell Sci. 130(8):1463-1474).

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Alias:
Testis-expressed protein 19.1;mTex19.1;Testis-expressed protein 19A

Gene Symbols:
Tex19.1;Tex19;Tex19a

Isotype:
IgG2b κ

Purification Method:
Protein G purified

Test Specificity:
Clone 7TEX-1F11 detects murine Testis-expressed protein 19.1 (Tex 19).

Clone:
7TEX-1F11

Applications:
ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Signaling

Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez):
NP_082878

Immunogen:
His-tagged full-length recombinant mouse Testis-expressed protein 19.1 (Tex 19).

Primary or Secondary:
Primary

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

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7162899

Mouse Monoclonal Antibody...


Antigen:
GFP

Dilution:
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
All species

Gene Alias:
Green fluorescent protein

Gene Symbols:
GFP

Isotype:
IgG1 κ

Purification Method:
Protein G purified

Test Specificity:
Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.

Clone:
2B6

Applications:
Immunocytochemistry, Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Secondary & Control Antibodies

Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez):
__

Immunogen:
His-tagged recombinant jellyfish Green fluorescent protein.

Primary or Secondary:
Primary

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

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Antigen:
Retinal Dehydrogenase 1/ALDH1A1

Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Retinal Dehydrogenase 1/ALDH1A1 in human liver and rat liver tissue sections.

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Rat

Gene Alias:
Retinal dehydrogenase 1;EC: 1.2.1.36;RALDH 1;RalDH1;ALDH-E1;ALHDII;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase;cytosolic

Gene Symbols:
ALDH1A;ALDC;ALDH1;PUMB1

Isotype:
IgG2b κ

Purification Method:
Protein G purified

Test Specificity:
Clone 16B4.2 detects Retinal dehydrogenase 1/ALDH1A1. it targets an epitope with in 18 ammo acids from the C-terminal region.

Clone:
16B4.2

Applications:
Immunohistochemistry (Paraffin), Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Neuroscience

Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene ID (Entrez):
NP_000680

Immunogen:
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human retinal dehydrogenase 1.

Primary or Secondary:
Primary

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

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7162902

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Antigen:
ESET (SETDB1)

Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Alias:
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1

Gene Symbols:
Setdb1;Eset;Kiaa0067

Isotype:
IgG1 κ

Purification Method:
Protein G purified

Test Specificity:
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.

Clone:
F0808

Applications:
ChIP Assay, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Epigenetics & Nuclear Function

Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.

Gene ID (Entrez):
__

Immunogen:
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.

Primary or Secondary:
Primary

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.