7162906
AsCpf1, Mouse anti-Bacteria, Clone: 3D3-F7, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
AsCpf1
Dilution
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Bacteria
Gene Alias
EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Immunogen
His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Clone
3D3-F7
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Cas12a;Cpf1
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Description
- Anti-AsCpf1, clone 3D3-F7, Cat
- No
- MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting
- CRISPR-associated endonuclease Cas12a (UniProt: U2UMQ6; also known as EC: 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1) I encoded by the Cas12a (also known as cpf1) gene in Acidaminococcus species
- CRISPR is an adaptive immune system that provides protection against mobile genetic elements, such as viruses, transposable elements, and conjugative plasmids
- CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids
- They are transcribed and processed into CRISPR RNA (crRNA)
- Cas12a, an endonuclease from the type V-A CRISPR system, has several unique characteristics when compared to Cas9 system that increase the utility of CRISPR-based gene editing
- Cas12a mediated cleavage utilizes a single and short CRISPR RNA) without requiring trans-activating crRNA
- Cas12a recognize a 5' T-rich protospacer adjacent motif (PAM, TTTN for this specific enzyme), unlike Cas9 enzymes which recognize 3' G-rich PAMs, thus this enzyme increases the possibilities for genome editing
- The endonuclease activity of Cas12a results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively.
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Antigen: AsCpf1
Dilution: Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Immunogen: His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 3D3-F7
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Cas12a;Cpf1
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Antigen:
AsCpf1
Dilution:
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Immunogen:
His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
3D3-F7
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Cas12a;Cpf1
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Antigen: beta-Catenin
Dilution: Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: beta-Catenin 1;Catenin beta-1
Immunogen: BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: mAb-Cat1
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Antigen:
beta-Catenin
Dilution:
Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
beta-Catenin 1;Catenin beta-1
Immunogen:
BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
mAb-Cat1
Applications:
ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Antigen: Histone H1
Dilution: ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Drosophila
Gene Alias: H1
Immunogen: GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: 1G6
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: His 1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Antigen:
Histone H1
Dilution:
ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Drosophila
Gene Alias:
H1
Immunogen:
GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
1G6
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
His 1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Antigen: BMAL1 (ARNTL)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Immunogen: MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Clone: B1BH2
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: ARNTL;BMAL1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Antigen:
BMAL1 (ARNTL)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Immunogen:
MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Clone:
B1BH2
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
ARNTL;BMAL1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.