7162910
Histone H1, Mouse anti-Drosophila, Clone: 1G6, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Histone H1
Dilution
ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Drosophila
Gene Alias
H1
Gene Symbols
His 1
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Clone
1G6
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001027286
Immunogen
GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-Histone H1, clone 1G6 Antibody, Cat
- No
- MABE1885, is a highly specific mouse monoclonal antibody that targets Drosophila Histone H1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting
- Histone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species
- Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation
- The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites
- Histone H1 is essential for the condensation of nucleosome chains into higher-order structures
- In metazoans, H1 histones are smaller in size with about 200 amino acids
- They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail
- The central globular domain is shown to be highly conserved among all H1 subtypes
- Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes
- Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding
- In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage
- Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs)
- Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis
- (Ref.: Hergeth, SP., and Schneider, R (2015)
- EMBO Rep
- 16(11); 1439-1453).
Compare Similar Items
Show Difference
Antigen: Histone H1
Dilution: ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Drosophila
Gene Alias: H1
Gene Symbols: His 1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Clone: 1G6
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001027286
Immunogen: GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Histone H1
Dilution:
ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Drosophila
Gene Alias:
H1
Gene Symbols:
His 1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Clone:
1G6
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001027286
Immunogen:
GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: BMAL1 (ARNTL)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols: ARNTL;BMAL1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone: B1BH2
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001001463
Immunogen: MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
BMAL1 (ARNTL)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols:
ARNTL;BMAL1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone:
B1BH2
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001001463
Immunogen:
MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: BMAL1 (ARNTL)
Dilution: Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols: ARNTL;BMAL1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone: B1BH2
Applications: ChIP Assay, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001001463
Immunogen: MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
BMAL1 (ARNTL)
Dilution:
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Aryl hydrocarbon receptor nuclear translocator-like protein 1;BMAL1b';Brain and muscle ARNT-like 1
Gene Symbols:
ARNTL;BMAL1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.
Clone:
B1BH2
Applications:
ChIP Assay, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001001463
Immunogen:
MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: Lap2alpha
Dilution: Western Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: Lamina-associated polypeptide 2 isoform alpha;Thymopoietin isoforms alpha;TP alpha
Gene Symbols: Tmpo;Lap2
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: Clone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region.
Clone: 8C10-1H11
Applications: ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Inflammation & Immunology
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_035735
Immunogen: His-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
Lap2alpha
Dilution:
Western Blotting Analysis: A representative lot detected Lap2alpha in Western Blotting applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Lap2alpha in Chromatin Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunoprecipitation Analysis: A representative lot immunoprecipitated Lap2alpha in Immunoprecipitation applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).Immunofluorescence Analysis: A representative lot detected Lap2alpha in Immunofluorescence applications (Gesson, K., et. al. (2016). Genome Res. 26(4):462-73).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Alias:
Lamina-associated polypeptide 2 isoform alpha;Thymopoietin isoforms alpha;TP alpha
Gene Symbols:
Tmpo;Lap2
Isotype:
IgG2a κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8C10-1H11 specifically detects Lamina-associated polypeptide 2 alpha (LAP2alpha) in murine species. It targets an epitope in the internal region.
Clone:
8C10-1H11
Applications:
ChIP Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Inflammation & Immunology
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_035735
Immunogen:
His-tagged recombinant fragment corresponding to 507 amino acids from the C-terminal region.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.