7162961
PEG, Mouse anti-All, Clone: 6.3, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PEG
Dilution
ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
All species
Gene Alias
Polyethylene Glycol
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone
6.3
Applications
ELISA
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Signaling
Formulation
Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Immunogen
PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-PEG, clone 6.3, Cat
- No
- MABS1966, is a mouse monoclonal antibody that detects PEG-conjugated proteins and it hs been tested for use in ELISA
- Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution
- The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation
- Clone 6.3 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG
- It binds to a smaller epitope of the PEG backbone when compared to other antibodies
- Since it binds to a smaller PEG epitope (∽750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20
- Instead 0.05% CHAPS is a more suitable detergent when using this antibody.
Compare Similar Items
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Antigen: PEG
Dilution: ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Polyethylene Glycol
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone: 6.3
Applications: ELISA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Immunogen: PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
PEG
Dilution:
ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Polyethylene Glycol
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).
Clone:
6.3
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.
Immunogen:
PEG-conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ST8SIA1
Dilution: Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Isotype: IgM κ
Purification Method: Purified by ion-exchange chromatography
Test Specificity: Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Clone: HIC0-3C5
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Immunogen: Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ST8SIA1
Dilution:
Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Isotype:
IgM κ
Purification Method:
Purified by ion-exchange chromatography
Test Specificity:
Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Clone:
HIC0-3C5
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Immunogen:
Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: mPEG
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Methoxy polyethylene glycol
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).
Clone: 15-2b
Applications: ELISA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
Immunogen: Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
mPEG
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Methoxy polyethylene glycol
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).
Clone:
15-2b
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
Immunogen:
Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: mPEG
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: All species
Gene Alias: Methoxy polyethylene glycol
Isotype: IgG2b κ
Purification Method: Protein A purified
Test Specificity: Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).
Clone: 15-2b
Applications: ELISA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
Immunogen: Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
mPEG
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
All species
Gene Alias:
Methoxy polyethylene glycol
Isotype:
IgG2b κ
Purification Method:
Protein A purified
Test Specificity:
Clone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).
Clone:
15-2b
Applications:
ELISA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
Immunogen:
Methoxy PEG covalently conjugated to E.coli beta-glucuronidase.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.