7162966
SREBP-1, Mouse anti-Hamster, Canine, Human, Clone: 2A4, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SREBP-1
Dilution
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Hamster, Canine, Human
Gene Alias
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Gene Symbols
SREBF1;BHLHD1;SREBP1
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Clone
2A4
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Signaling
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001005291.1
Immunogen
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-SREBP-1, clone 2A4, Cat
- No
- MABS2034, is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Western Blotting
- Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human
- SREBP-1 is a multi-pass membrane protein found on nuclear, endoplasmic reticulum, and Golgi membranes
- It attaches to membranes via two hydrophobic sequences (aa 477-497 and 536-556)
- It is expressed in a wide variety of tissues, but is most abundant in liver and adrenal gland
- Six isoforms of SREBP-1 have been described that are produced by alternative splicing
- Isoform SREBP-1C predominates in liver, adrenal gland, and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines
- Both isoforms are found in kidney, brain, white fat, and muscle
- It serves as a transcriptional activator that is essential for lipid homeostasis and regulates transcription of the LDL receptor gene
- It is also involved in fatty acid and cholesterol synthesis
- It acts by binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') and displays dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')
- SREBP-1 has two cytoplasmic domains (aa 1-487 and 569-1147), two transmembrane domains (aa 88-508 and 548-568), and one lumenal domain (aa 509-547)
- In the precursor form, which has a hairpin orientation in the membrane both the N-terminal transcription factor domain and the C-terminal regulatory domain face the cytoplasm
- Two separate site-specific proteolytic cleavages are required for release of the transcriptionally active N-terminal domain
- These cleavages are carried out by two distinct proteases, called site-1 protease (S1P; aa 490-491) and site-2 protease (S2P; aa 530-531)
- SREBP-1 can be phosphorylated by AMPK at Ser402, which suppress its processing and nuclear translocation, and thereby repress target gene expression.
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Antigen: SREBP-1
Dilution: Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Hamster, Canine, Human
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Gene Symbols: SREBF1;BHLHD1;SREBP1
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Clone: 2A4
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Signaling
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001005291.1
Immunogen: His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
SREBP-1
Dilution:
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Hamster, Canine, Human
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Clone:
2A4
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Signaling
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001005291.1
Immunogen:
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: GCP2
Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represneta
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Chicken, Human, Frog, Fish
Gene Alias: Gamma-tubulin complex component 2
Gene Symbols: Tubgcp2;Gcp2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
Clone: GCP2-02
Applications: ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001272936.1
Immunogen: GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
GCP2
Dilution:
Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represneta
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Chicken, Human, Frog, Fish
Gene Alias:
Gamma-tubulin complex component 2
Gene Symbols:
Tubgcp2;Gcp2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
Clone:
GCP2-02
Applications:
ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001272936.1
Immunogen:
GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ADAM 12
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone: 8F8
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone:
8F8
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ADAM 12
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone: 6E6
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone:
6E6
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.