7162970
GCP2, Mouse anti-Mouse, Chicken, Human, Frog, Fish, Clone: GCP2-02, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
GCP2
Dilution
Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represneta
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse, Chicken, Human, Frog, Fish
Gene Alias
Gamma-tubulin complex component 2
Gene Symbols
Tubgcp2;Gcp2
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
Clone
GCP2-02
Applications
ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cell Structure
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001272936.1
Immunogen
GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Description
- Anti-GCP2, clone GCP2-02, Cat
- No
- MABT1322, is a mouse monoclonal antibody that detects Gamma-tubulin complex component 2 (CGP2) and has been tested for use in ELISA, Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting
- Gamma-tubulin complex component 2 (UniProt: Q921G8; also known as GCP2) is encoded by the Tubgcp2 (also known as Gcp2) gene (Gene ID: 74237) in murine species
- Gamma-tubulin complex that is essential for microtubule nucleation at the centrosome is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6
- GCPs are characterized by sequence homology in two specific regions known as the grip1 and grip2 motifs
- GPCs associate with gamma-tubulin to form a V-shaped subcomplex known as gamma-tubulin small complex
- In this complex GCPs 2 and 3 interact via their N-terminal domain and form the arms of the V
- The two-distal part of the V (C-terminal domains) bind to gamma-tubulin
- The gamma-tubulin complex serves as a template for polarized growth and regulation of microtubules in eukaryotes
- Significantly higher expression of both GPC2 and 3 have been reported in glioblastoma cells and they are concentrated I the centrosomes in interphase glioblastoma cells
- Depletion of GPC2 and 3 causes accumulation of cells in G2/M and result in mitotic delay
- (Ref.: Farache, D., et al
- (2016)
- J
- Biol
- Chem
- 291(44); 23112-25; Draberova, E., et al
- (2015)
- J
- Neuropathol
- Exp
- Neurol
- 74(7); 723-42).
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Antigen: GCP2
Dilution: Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represneta
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Chicken, Human, Frog, Fish
Gene Alias: Gamma-tubulin complex component 2
Gene Symbols: Tubgcp2;Gcp2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
Clone: GCP2-02
Applications: ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001272936.1
Immunogen: GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
GCP2
Dilution:
Western Blotting Analysis: 1μg/mL from a representative lot detected GCP2 in human glioblastoma T98G cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated GCP2 in Immunoprecipitation applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Immunocytochemistry Analysis: A representative lot detected GCP2 in Immunocytochemistry applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Western Blotting Analysis: A representative lot detected GCP2 in Western Blotting applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).ELISA Analysis: A representative lot detected GCP2 in ELISA applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42).Electron Microscopy Analysis: A representative lot detected GCP2 in Electron Microscopy applications (Draberova, E., et. al. (2015). J Neuropathol Exp Neurol. 74(7):723-42). Immunoprecipitation Analysis: A represneta
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Chicken, Human, Frog, Fish
Gene Alias:
Gamma-tubulin complex component 2
Gene Symbols:
Tubgcp2;Gcp2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone GCP2-02 specifically detects GCP2 in T98G glioblastoma cells. It targets an epitope within 12 amino acids from the N-terminal region.
Clone:
GCP2-02
Applications:
ELISA, Immunocytochemistry, Immunomicroscopy, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001272936.1
Immunogen:
GST-tagged recombinant fragment corresponding to the first 194 amino acids from the N-terminal region of murine Gamma-tubulin complex component 2.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ADAM 12
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone: 8F8
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).Inhibition Analysis: A representative inhibited ADAM 12 induced gelatin degradation. (Albrechtsen, R., et. al. (2013). J Cell Sci. 126(Pt 20):4707-20). Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications (Sundberg, C., et. al. (2004). J Biol Chem. 279(49):51601-11).Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications (Kveiborg, M., et. al. (2005). Cancer Res. 65(11):4754-61).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 8F8 detects ADAM 12-DeltaCyt secreted form in human cells. It targets an epitope with in the prodomain region.
Clone:
8F8
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: ADAM 12
Dilution: Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols: ADAM12;MLTN;UNQ346/PRO545
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone: 6E6
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis & Cancer
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001275903
Immunogen: A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
ADAM 12
Dilution:
Flow Cytometry Analysis: 1μg from a representative lot detected ADAM 12 in one million ADAM 12-DeltaCyt MCF-7 cells.Western Blotting Analysis: A representative lot detected ADAM 12 in Western Blotting applications.Immunoprecipitation Analysis: A representative lot immunoprecipitated ADAM 12 in Immunoprecipitation applications.Immunocytochemistry Analysis: A representative lot detected ADAM 12 in Immunocytochemistry applications.Flow Cytometry Analysis: A representative lot detected ADAM 12 in Flow cytometry applications.
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha
Gene Symbols:
ADAM12;MLTN;UNQ346/PRO545
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 6E6 detects ADAM 12-DeltaCyt. It targets an epitope with in extracellular disintegrin-cysteine-rich domains.
Clone:
6E6
Applications:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis & Cancer
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001275903
Immunogen:
A full-length recombinant human ADAM12 secreted form expressed in HEK293 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: pan-Actin
Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone: 2A3
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001092
Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
pan-Actin
Dilution:
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
Gene Alias:
Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
Gene Symbols:
ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
Clone:
2A3
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001092
Immunogen:
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.