7162983
PD2 (PAF1), Mouse anti-Human, Clone: F169-3B2, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PD2 (PAF1)
Dilution
Immunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91).Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Pancreatic differentiation protein 2;RNA polymerase II-associated factor 1 homolog;hPAF1
Gene Symbols
PAF1;PD2
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half.
Clone
F169-3B2
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez)
NP_061961
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Description
- Anti-PD2 (PAF1), clone F169-3B2, Cat
- No
- MABT1495, is mouse monoclonal antibody that detects Pancreatic differentiation protein 2 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting
- Pancreatic differentiation protein 2 (UniProt: Q8N7H5; also known as RNA polymerase II-associated factor 1 homolog, PD2, hPAF1) is encoded by the PAF1 (also known as PD2) gene (Gene ID: 54623) in human
- PD2 is a nuclear protein that serves as a subunit of the RNA polymerase II associated factor (PAF) complex
- It has multiple functions during transcription by RNA polymerase II
- It has been implicated in regulation of development and maintenance of embryonic stem cell pluripotency
- It associates with RNA polymerase II through interaction with POLR2A C-terminal domain non-phosphorylated and serine 2 and serine 5 phosphorylated forms
- It is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1
- Three isoforms of PD2 have been described that are produced by alternative splicing
- PD2 has oncogenic property and is shown to be overexpressed in pancreatic cancer cells
- In normal murine pancreas, its expression is restricted to acinar cells, however, its expression is increased in the ductal cells of mouse model of pancreatic cancer with advancing age
- (Ref.: Dey, P., et al (2014)
- Oncotarget, 5(12); 4480-4491).
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Show Difference
Antigen: PD2 (PAF1)
Dilution: Immunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91).Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Pancreatic differentiation protein 2;RNA polymerase II-associated factor 1 homolog;hPAF1
Gene Symbols: PAF1;PD2
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half.
Clone: F169-3B2
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez): NP_061961
Immunogen: KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen:
PD2 (PAF1)
Dilution:
Immunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91).Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Pancreatic differentiation protein 2;RNA polymerase II-associated factor 1 homolog;hPAF1
Gene Symbols:
PAF1;PD2
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half.
Clone:
F169-3B2
Applications:
ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene ID (Entrez):
NP_061961
Immunogen:
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Antigen: P40 (TP63)
Dilution: __
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;TP63;Tumor protein p73-like;p73L;P51
Gene Symbols: P63;KET;P63;P73H;P73L;TP73L
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 11H1 detects human Tumor protein 63 (P40). It targets an epitope with in the first 205 amino acids from the N-terminal region.
Clone: 11H1
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Epigenetics & Nuclear Function
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001108452
Immunogen: MBP-conjugated recombinant fragment corresponding to the first 205 amino acids from human Tumor protein 63.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
P40 (TP63)
Dilution:
__
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;TP63;Tumor protein p73-like;p73L;P51
Gene Symbols:
P63;KET;P63;P73H;P73L;TP73L
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 11H1 detects human Tumor protein 63 (P40). It targets an epitope with in the first 205 amino acids from the N-terminal region.
Clone:
11H1
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Epigenetics & Nuclear Function
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001108452
Immunogen:
MBP-conjugated recombinant fragment corresponding to the first 205 amino acids from human Tumor protein 63.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: phospho-cytokeratin-8 (K8) (Ser431)
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols: KRT8;CYK8
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Clone: 5B3
Applications: Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001243222
Immunogen: Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
phospho-cytokeratin-8 (K8) (Ser431)
Dilution:
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Immunofluorescence applications (Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser431) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16; Ridge, K.M., et. al. (2005). J Biol Chem. 280(34):30400-5; Ku, N.O., et. al. (1997). J Biol Chem. 272(11):7556-64).
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Alias:
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols:
KRT8;CYK8
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone 5B3 specifically detects cytokeratin-8 phosphorylated on serine 431.
Clone:
5B3
Applications:
Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001243222
Immunogen:
Cytokeratin-8/18 purified from okadaic acid-treated HT29 cells.
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Antigen: phospho-cytokeratin-8 (K8) (Ser73)
Dilution: Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Human
Gene Alias: Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols: KRT8;CYK8
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Clone: LJ4
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Structure
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez): NP_001243222
Immunogen: Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Primary or Secondary: Primary
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Antigen:
phospho-cytokeratin-8 (K8) (Ser73)
Dilution:
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Human
Gene Alias:
Keratin;type II cytoskeletal 8;CK-8;Keratin-8;K8;Type-II keratin Kb80
Gene Symbols:
KRT8;CYK8
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.
Clone:
LJ4
Applications:
ELISA, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cell Structure
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez):
NP_001243222
Immunogen:
Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).
Primary or Secondary:
Primary
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.