7188644
Histone H2A.X Polyclonal Antibody, Active Motif™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Histone H2A.X
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human
Gene Accession No.
P16104
Gene ID (Entrez)
3014
Immunogen
This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
Primary or Secondary
Primary
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Gene
H2AX
Concentration
Lot-Specific
Classification
Polyclonal
Form
Liquid
Regulatory Status
RUO
Formulation
70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
Gene Alias
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2a.x; H2a/x; h2afx; H2AX; H2AX histone; Hist5-2ax; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Gene Symbols
H2AX
Isotype
IgG
Purification Method
Antigen affinity chromatography
Product Type
Antibody
Related Products
Description
- Description Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle
- In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX
- The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs
- The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner
- Structurally, H2A.x contains 143 amino acid residues
- Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs
- There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA
- The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA
- Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome
- Histone H2A.x contributes to histone-formation and therefore the structure of DNA
- Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
Compare Similar Items
Show Difference
Antigen: Histone H2A.X
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: P16104
Gene ID (Entrez): 3014
Immunogen: This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Gene: H2AX
Concentration: Lot-Specific
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
Gene Alias: AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2a.x; H2a/x; h2afx; H2AX; H2AX histone; Hist5-2ax; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Gene Symbols: H2AX
Isotype: IgG
Purification Method: Antigen affinity chromatography
Product Type: Antibody
Antigen:
Histone H2A.X
Applications:
Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
P16104
Gene ID (Entrez):
3014
Immunogen:
This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Gene:
H2AX
Concentration:
Lot-Specific
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
Gene Alias:
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2a.x; H2a/x; h2afx; H2AX; H2AX histone; Hist5-2ax; histone 5 protein 2ax; Histone H2A.X; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Gene Symbols:
H2AX
Isotype:
IgG
Purification Method:
Antigen affinity chromatography
Product Type:
Antibody
Antigen: HIF-1 alpha
Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: Q16665
Gene ID (Entrez): 3091
Immunogen: This HIF-1 alpha antibody was raised against a synthetic peptide corresponding to the C-terminal region of human HIF-1 alpha.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Gene: HIF1A
Concentration: Conc. Not Determined
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: whole serum with 30% glycerol and 0.035% sodium azide; pH
Gene Alias: AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; BOS_11009; Class E basic helix-loop-helix protein 78; HIF1; HIF1 alpha; HIF-1 alpha; hif1a; HIF-1A; hif1a.L; HIF1alpha; hif-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS
Gene Symbols: HIF1A
Isotype: IgG
Purification Method: __
Product Type: Antibody
Antigen:
HIF-1 alpha
Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
Q16665
Gene ID (Entrez):
3091
Immunogen:
This HIF-1 alpha antibody was raised against a synthetic peptide corresponding to the C-terminal region of human HIF-1 alpha.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Gene:
HIF1A
Concentration:
Conc. Not Determined
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
whole serum with 30% glycerol and 0.035% sodium azide; pH
Gene Alias:
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; BOS_11009; Class E basic helix-loop-helix protein 78; HIF1; HIF1 alpha; HIF-1 alpha; hif1a; HIF-1A; hif1a.L; HIF1alpha; hif-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS
Gene Symbols:
HIF1A
Isotype:
IgG
Purification Method:
__
Product Type:
Antibody
Antigen: FOXO1/FKHR
Applications: Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: Q12778
Gene ID (Entrez): 2308
Immunogen: This FOXO1/FkHR antibody was raised against a peptide from the C-terminal region of human FOXO1/FkHR.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Gene: FOXO1
Concentration: Lot-Specific
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
Gene Alias: Afxh; AI876417; FKH1; FKHR; Fkhr1; forkhead box O1; forkhead box O1 a; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O1-A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; fox family transcription factor FoxO1a.1; FOX01A; foxhead box protein O1-A; Foxo1; Foxo1a; foxO1a.1; zgc:153388
Gene Symbols: FOXO1
Isotype: IgG
Purification Method: Antigen affinity chromatography
Product Type: Antibody
Antigen:
FOXO1/FKHR
Applications:
Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
Q12778
Gene ID (Entrez):
2308
Immunogen:
This FOXO1/FkHR antibody was raised against a peptide from the C-terminal region of human FOXO1/FkHR.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Gene:
FOXO1
Concentration:
Lot-Specific
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
Gene Alias:
Afxh; AI876417; FKH1; FKHR; Fkhr1; forkhead box O1; forkhead box O1 a; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O1-A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; fox family transcription factor FoxO1a.1; FOX01A; foxhead box protein O1-A; Foxo1; Foxo1a; foxO1a.1; zgc:153388
Gene Symbols:
FOXO1
Isotype:
IgG
Purification Method:
Antigen affinity chromatography
Product Type:
Antibody
Antigen: Mi-2
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Drosophila
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acid residues 1641-1662 of Drosophila Mi-2.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Gene: __
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Gene Alias: 9530019N15Rik; AA617397; ATP-dependent helicase CHD4; BC005710; CHD4; CHD-4; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; D6Ertd380e; Mi-2; Mi-2 autoantigen; Mi-2 autoantigen 218 kDa protein; Mi-2 beta; MI2B; Mi-2b; Mi-2beta; Mi2-beta; mKIAA4075
Gene Symbols: __
Isotype: IgG2c
Purification Method: Protein G
Product Type: Antibody
Antigen:
Mi-2
Applications:
Immunofluorescence, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Drosophila
Gene Accession No.:
__
Gene ID (Entrez):
__
Immunogen:
This antibody was raised against a synthetic peptide corresponding to amino acid residues 1641-1662 of Drosophila Mi-2.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Gene:
__
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Gene Alias:
9530019N15Rik; AA617397; ATP-dependent helicase CHD4; BC005710; CHD4; CHD-4; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; D6Ertd380e; Mi-2; Mi-2 autoantigen; Mi-2 autoantigen 218 kDa protein; Mi-2 beta; MI2B; Mi-2b; Mi-2beta; Mi2-beta; mKIAA4075
Gene Symbols:
__
Isotype:
IgG2c
Purification Method:
Protein G
Product Type:
Antibody