7188681
His-Tag Recombinant Mouse Monoclonal Antibody, AbFlex™, Active Motif™
Mouse Recombinant Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
His-Tag
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Formulation
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Immunogen
The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Primary or Secondary
Primary
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Isotype
IgG2a
Purification Method
Protein A
Product Type
Antibody
Related Products
Description
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
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Antigen: His-Tag
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Formulation: 140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Immunogen: The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Isotype: IgG2a
Purification Method: Protein A
Product Type: Antibody
Antigen:
His-Tag
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Formulation:
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Immunogen:
The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Isotype:
IgG2a
Purification Method:
Protein A
Product Type:
Antibody
Antigen: Phospho-RNA pol II CTD (Thr4)
Applications: Dot Blot, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen: This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: Lot-Specific
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 220kDa; BOS_18773; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; Pol II S2p; POLR2; POLR2A; POLRA; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8
Isotype: IgG
Purification Method: Protein A
Product Type: Antibody
Antigen:
Phospho-RNA pol II CTD (Thr4)
Applications:
Dot Blot, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen:
This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
Lot-Specific
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
220kDa; BOS_18773; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; Pol II S2p; POLR2; POLR2A; POLRA; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8
Isotype:
IgG
Purification Method:
Protein A
Product Type:
Antibody
Antigen: Phospho-RNA pol II CTD (Thr4)
Applications: Dot Blot, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen: This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: Lot-Specific
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 220kDa; BOS_18773; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; Pol II S2p; POLR2; POLR2A; POLRA; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8
Isotype: IgG
Purification Method: Protein A
Product Type: Antibody
Antigen:
Phospho-RNA pol II CTD (Thr4)
Applications:
Dot Blot, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen:
This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
Lot-Specific
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
220kDa; BOS_18773; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; Pol II S2p; POLR2; POLR2A; POLRA; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II 1; RNA polymerase II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; Rpb1; RPB220; RPBh1; Rpii215; RpIILS; RPO2; RPO21; Rpo2-1; RPOL2; SUA8
Isotype:
IgG
Purification Method:
Protein A
Product Type:
Antibody
Antigen: DDB1
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen: This DDB1 antibody was raised against a peptide from the C-terminus of human DDB1.
Primary or Secondary: Primary
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: Lot-Specific
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 127kDa; AA408517; damage specific DNA binding protein 1; damaged-DNA recognition protein 1; damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; DDB p127 subunit; DDB1; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; p127-Ddb1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE; XPE-BF; XPE-binding factor
Isotype: IgG
Purification Method: Antigen affinity chromatography
Product Type: Antibody
Antigen:
DDB1
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
Immunogen:
This DDB1 antibody was raised against a peptide from the C-terminus of human DDB1.
Primary or Secondary:
Primary
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
Lot-Specific
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
127kDa; AA408517; damage specific DNA binding protein 1; damaged-DNA recognition protein 1; damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; DDB p127 subunit; DDB1; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; p127-Ddb1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE; XPE-BF; XPE-binding factor
Isotype:
IgG
Purification Method:
Antigen affinity chromatography
Product Type:
Antibody