alpha-Tubulin Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein
• A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence
• Cross reactivity will occur with all isoforms of alpha tubulin
• Such broad reactivity makes this antibody useful as an excellent loading control
• Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits
• The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical
• Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells
• Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis
• Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins
• The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly
• There are multiple alpha and beta tubulin genes, which are highly conserved among species
• The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene
• Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.