7190876
L1/ORF2 Polyclonal Antibody, Rockland™
Chicken Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
L1/ORF2
Applications
ELISA, Western Blot
Conjugate
Unconjugated
Host Species
Chicken
Target Species
Chimpanzee, Human
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Concentration
1 mg/mL
Classification
Polyclonal
Form
Liquid
Regulatory Status
RUO
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary
Primary
Product Type
Antibody
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein
- Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays
- A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences
- The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies
- This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling
- Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition
- ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains
- The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias
- This antibody is intended for use in studying control of L1 retrotransposons.
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Antigen: L1/ORF2
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Chicken
Target Species: Chimpanzee, Human
Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: 1 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
L1/ORF2
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Host Species:
Chicken
Target Species:
Chimpanzee, Human
Immunogen:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
1 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: HDAC1 (C-terminus)
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Immunogen: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: 1.33 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
HDAC1 (C-terminus)
Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Immunogen:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
1.33 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: HDAC1 (C-terminus)
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Immunogen: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: 1.33 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
HDAC1 (C-terminus)
Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Immunogen:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
1.33 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: Wnt1
Applications: ELISA, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Immunogen: Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
Wnt1
Applications:
ELISA, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Immunogen:
Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody