7190999

DYKDDDDK Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

DYKDDDDK

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Host Species

Rabbit

Formulation

0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary

Primary

Product Type

Antibody

Concentration

1.2 mg/mL

Classification

Polyclonal

Form

Liquid

Regulatory Status

RUO

Gene Alias

DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

Content And Storage

-20°C, Avoid Freeze/Thaw Cycles

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Description

  • Store vial at -20°C prior to opening
  • Aliquot contents and freeze at -20°C or below for extended storage
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • This affinity purified antibody is directed against the FLAGTM motif and is useful in determining its presence in various assays
  • This polyclonal anti-FLAGTM tag antibody detects over-expressed proteins containing the FLAGTM epitope tag
  • In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
  • DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
  • The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
  • A FLAG tag can be used in many different assays that require recognition by an antibody
  • If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
  • Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
  • Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
  • The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
  • The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
  • FLAG is a trademark of Sigma-Aldrich Biotechnology LP

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Show Difference

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Fischer Scientific

7190999

Rabbit Polyclonal Antibody...


Antigen:
DYKDDDDK

Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1.2 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles

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7191000

Mouse Monoclonal Antibody...


Antigen:
AKT1

Applications:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Mouse

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Alias:
Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein ki

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles

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7191004

Rabbit Polyclonal Antibody...


Antigen:
THRA

Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Alias:
6430529J03Rik; AR7; avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1; AW259572; cerbA protein; c-erb-A protein; c-erb-A thyroid hormone receptor; c-erbA-1; cerbAalpha; c-erbAalpha; C-erba-alpha; c-erbAalpha2; c-erbA-alpha2; c-erbAalpha2; thyroid hormone receptor alpha; CHNG6; E ERB-T-1; EAR7; EAR-7; ERBA; ERBA ALPHA; ERBA related 7; ERBA1; ERBA-related 7; ERB-T-1; MGC000261; MGC43240; Nr1a1; Nuclear receptor subfamily 1 group A member 1; Oncogene ERBA; Rvr; T3R[a]; T3Ralpha; THRA; THRA1; THRA2; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1 formerly ERBA1); thyroid hormone receptor alpha 2; thyroid hormone receptor, alpha; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; TR; TR alpha 1; TR alpha 1+2; TR alpha 2; triiodothyronine receptor; V-erbA-relat

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles

Img

Fischer Scientific

7191004

Rabbit Polyclonal Antibody...


Antigen:
THRA

Applications:
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Alias:
6430529J03Rik; AR7; avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1; AW259572; cerbA protein; c-erb-A protein; c-erb-A thyroid hormone receptor; c-erbA-1; cerbAalpha; c-erbAalpha; C-erba-alpha; c-erbAalpha2; c-erbA-alpha2; c-erbAalpha2; thyroid hormone receptor alpha; CHNG6; E ERB-T-1; EAR7; EAR-7; ERBA; ERBA ALPHA; ERBA related 7; ERBA1; ERBA-related 7; ERB-T-1; MGC000261; MGC43240; Nr1a1; Nuclear receptor subfamily 1 group A member 1; Oncogene ERBA; Rvr; T3R[a]; T3Ralpha; THRA; THRA1; THRA2; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1 formerly ERBA1); thyroid hormone receptor alpha 2; thyroid hormone receptor, alpha; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid normone nuclear receptor alpha variant 1; TR; TR alpha 1; TR alpha 1+2; TR alpha 2; triiodothyronine receptor; V-erbA-relat

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles