50-199-1714
6X His Epitope Tag Polyclonal Antibody, Rockland™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-1714-Each-of-1 | In Stock | ₹ 31,212.29 |
50-199-1714 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,212.29
GST (18%): ₹ 5,618.212
Total Price: ₹ 36,830.502
Antigen
6X His Epitope Tag
Classification
Polyclonal
Conjugate
Unconjugated
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Quantity
100 μg
Primary or Secondary
Primary
Product Type
Antibody
Target Molecule
Tag
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration
1.1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Rabbit
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
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Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays
- This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Compare Similar Items
Show Difference
Antigen: 6X His Epitope Tag
Classification: Polyclonal
Conjugate: Unconjugated
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Target Molecule: Tag
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration: 1.1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Antigen:
6X His Epitope Tag
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Target Molecule:
Tag
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration:
1.1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Antigen: 6X His Epitope Tag
Classification: Polyclonal
Conjugate: Unconjugated
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Target Molecule: Tag
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration: 1.1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Antigen:
6X His Epitope Tag
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Target Molecule:
Tag
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration:
1.1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Antigen: DYKDDDDK
Classification: Polyclonal
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Quantity: 250 μg
Primary or Secondary: Primary
Product Type: Antibody
Target Molecule: Tag
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration: 1.2 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Antigen:
DYKDDDDK
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Quantity:
250 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Target Molecule:
Tag
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration:
1.2 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Antigen: DYKDDDDK
Classification: Polyclonal
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Target Molecule: Tag
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration: 1.2 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Rabbit
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Antigen:
DYKDDDDK
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Target Molecule:
Tag
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Concentration:
1.2 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Rabbit
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid