50-199-0522
6X His Epitope Tag Monoclonal Antibody (33D10.D2.G8), Rockland™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0522-Each-of-1 | In Stock | ₹ 14,374.08 |
50-199-0522 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,374.08
GST (18%): ₹ 2,587.334
Total Price: ₹ 16,961.414
Antigen
6X His Epitope Tag
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
25 μL
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone
33D10.D2.G8
Conjugate
Unconjugated
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Target Molecule
Tag
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- Directed against the 6X His motif and is useful in determining its presence in various assays
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Compare Similar Items
Show Difference
Antigen: 6X His Epitope Tag
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone: 33D10.D2.G8
Conjugate: Unconjugated
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
6X His Epitope Tag
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Clone:
33D10.D2.G8
Conjugate:
Unconjugated
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: DYKDDDDK
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 29E4.G7
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
DYKDDDDK
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
29E4.G7
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: DYKDDDDK
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 25 μL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 29E4.G7
Conjugate: Unconjugated
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
DYKDDDDK
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
25 μL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
29E4.G7
Conjugate:
Unconjugated
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: Insulin
Classification: Monoclonal
Concentration: 1 mg/mL
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Applications: Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 2D11.H5
Conjugate: Unconjugated
Gene Alias: AA986540; BOS_25069; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; Insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin
Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Insulin
Classification:
Monoclonal
Concentration:
1 mg/mL
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Applications:
Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
2D11.H5
Conjugate:
Unconjugated
Gene Alias:
AA986540; BOS_25069; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; Insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin
Immunogen:
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__