7191017
6X His Epitope Tag Monoclonal Antibody (33D10.D2.G8), Rockland™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
6X His Epitope Tag
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Isotype
IgG1 κ
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Clone
33D10.D2.G8
Applications
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Primary or Secondary
Primary
Product Type
Antibody
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- Directed against the 6X His motif and is useful in determining its presence in various assays
- This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
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Antigen: 6X His Epitope Tag
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Isotype: IgG1 κ
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: 33D10.D2.G8
Applications: ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
6X His Epitope Tag
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Isotype:
IgG1 κ
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
33D10.D2.G8
Applications:
ELISA, Fluorescence Resonance Energy Transfer (FRET), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: Kinesin-1
Concentration: 77 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: AL022807; Conventional kinesin heavy chain; EMBL:ABC25059.1}; epididymis secretory protein Li 61; HEL-S-61; Khc; Khcs; KIF5B; kif5b {ECO:0000312; kinesin 1 (110-120kD); kinesin family member 5B; kinesin heavy chain; kinesin heavy chain member 5B, ubiquitous; Kinesin-1 heavy chain; KINH; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC; U-KHC
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
Kinesin-1
Concentration:
77 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
AL022807; Conventional kinesin heavy chain; EMBL:ABC25059.1}; epididymis secretory protein Li 61; HEL-S-61; Khc; Khcs; KIF5B; kif5b {ECO:0000312; kinesin 1 (110-120kD); kinesin family member 5B; kinesin heavy chain; kinesin heavy chain member 5B, ubiquitous; Kinesin-1 heavy chain; KINH; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC; U-KHC
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: beta Galactosidase
Concentration: 1 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: Acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; Glb1; Glb-1; Lactase; lysosomal beta-galactosidase; MPS4B; ßgal; ß-gal; ß-galactosidase
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: Dot Blot, ELISA, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Chicken
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
beta Galactosidase
Concentration:
1 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
Acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; Glb1; Glb-1; Lactase; lysosomal beta-galactosidase; MPS4B; ßgal; ß-gal; ß-galactosidase
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
Dot Blot, ELISA, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Chicken
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli
Primary or Secondary:
Primary
Product Type:
Antibody
Antigen: Osteopontin
Concentration: 95 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 2AR; 2b7; 44 kDa bone phosphoprotein; Apl-1; BNSP; Bone sialoprotein 1; Bsp; BSPI; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; Eta; Eta-1; HGNC:11255; MGC110940; Minopontin; Nephropontin; Op; OPN; Opnl; OSP; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; osteopontin-like protein; PSEC0156; Ric; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); Spp1; Spp-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin
Isotype: __
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Clone: __
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Primary or Secondary: Primary
Product Type: Antibody
Antigen:
Osteopontin
Concentration:
95 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
2AR; 2b7; 44 kDa bone phosphoprotein; Apl-1; BNSP; Bone sialoprotein 1; Bsp; BSPI; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; Eta; Eta-1; HGNC:11255; MGC110940; Minopontin; Nephropontin; Op; OPN; Opnl; OSP; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; osteopontin-like protein; PSEC0156; Ric; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); Spp1; Spp-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin
Isotype:
__
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Clone:
__
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Immunogen:
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
Primary or Secondary:
Primary
Product Type:
Antibody