7191020

beta Galactosidase Polyclonal Antibody, Rockland™

Chicken Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

beta Galactosidase

Applications

Dot Blot, ELISA, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Host Species

Chicken

Target Species

Bacteria

Gene Alias

Acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; Glb1; Glb-1; Lactase; lysosomal beta-galactosidase; MPS4B; ßgal; ß-gal; ß-galactosidase

Primary or Secondary

Primary

Product Type

Antibody

Concentration

1 mg/mL

Classification

Polyclonal

Form

Liquid

Regulatory Status

RUO

Formulation

0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Immunogen

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli

Content And Storage

-20°C, Avoid Freeze/Thaw Cycles

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Description

  • Store vial at -20°C prior to opening
  • Aliquot contents and freeze at -20°C or below for extended storage
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and anti-Chicken Serum
  • Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined
  • Very low background staining has been reported in various assays
  • Beta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety
  • Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome
  • In E
  • coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low
  • E
  • coli beta Galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression
  • Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones
  • Beta Galactosidase can be split in two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme
  • This property is exploited in many cloning vectors
  • The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others
  • Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.

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Antigen:
beta Galactosidase

Applications:
Dot Blot, ELISA, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Chicken

Target Species:
Bacteria

Gene Alias:
Acid beta-galactosidase; AW125515; betagal; Beta-Gal; Beta-galactosidase; bgal; Bge; Bgl; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik; EBP; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; Glb1; Glb-1; Lactase; lysosomal beta-galactosidase; MPS4B; ßgal; ß-gal; ß-galactosidase

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Immunogen:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles

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Antigen:
Osteopontin

Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Canine, Human, Mouse, Porcine, Rat

Gene Alias:
2AR; 2b7; 44 kDa bone phosphoprotein; Apl-1; BNSP; Bone sialoprotein 1; Bsp; BSPI; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; Eta; Eta-1; HGNC:11255; MGC110940; Minopontin; Nephropontin; Op; OPN; Opnl; OSP; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; osteopontin-like protein; PSEC0156; Ric; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); Spp1; Spp-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
95 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Immunogen:
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.

Content And Storage:
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Antigen:
Osteopontin

Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Canine, Human, Mouse, Porcine, Rat

Gene Alias:
2AR; 2b7; 44 kDa bone phosphoprotein; Apl-1; BNSP; Bone sialoprotein 1; Bsp; BSPI; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; Eta; Eta-1; HGNC:11255; MGC110940; Minopontin; Nephropontin; Op; OPN; Opnl; OSP; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; osteopontin-like protein; PSEC0156; Ric; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); Spp1; Spp-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
95 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Immunogen:
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.

Content And Storage:
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Antigen:
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Applications:
ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Human

Gene Alias:
1110034C02Rik; acute phase response factor; Acute-phase response factor; ADMIO; ADMIO1; APRF; AW109958; BOS_19088; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transduction and activation of transcription 3; STAT; STAT3; STAT-3; stat3 protein; STAT-3 protein; STAT3b1; STAT3b2; transcription factor; wu:fc15d02; wu:fl59g06; z-Stat3

Primary or Secondary:
Primary

Product Type:
Antibody

Concentration:
1 mg/mL

Classification:
Polyclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Immunogen:
__

Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles