Phospho-STAT3 (Tyr705) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-STAT3 antibody is an affinity-purified antibody and is directed against the phosphorylated form of human STAT3 protein at the Y705
• Anti-Stat3 antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated Stat3 protein is minimal by ELISA and western blot
• A BLAST analysis was used to suggest cross reactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen
• Reactivity against homologues from other sources is not known
• STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors
• Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3
• STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6
• Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity
• In humans, the STAT3 gene is located on the q arm of chromosome 17
• STAT3 has been shown to be activated by IFN-alpha but not IFN-beta
• The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB)
• STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis
• The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3
• Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described
• Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.