7191130
6X His Epitope Tag Polyclonal Antibody, Rockland™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
6X His Epitope Tag
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary
Primary
Product Type
Antibody
Concentration
1.1 mg/mL
Classification
Polyclonal
Form
Liquid
Regulatory Status
RUO
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
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Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays
- This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag
- To date, this antibody has reacted with all His tagged proteins so far tested
- In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins
- The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
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Antigen: 6X His Epitope Tag
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Concentration: 1.1 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Antigen:
6X His Epitope Tag
Applications:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Concentration:
1.1 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Antigen: Scythe/Bat3
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Concentration: 80 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 2410045D21Rik; AA408914; BAG 6; BAG family molecular chaperone regulator 6; BAG6; BAG-6; BAT 3; Bat3; BCL2 associated athanogene 6; BCL2-associated athanogene 6; D17H6S52E; D6S52E; DAQB-195H10.3; G3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; large proline-rich protein BAT3; Protein G3; Protein Scythe; Scythe
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Antigen:
Scythe/Bat3
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Concentration:
80 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
2410045D21Rik; AA408914; BAG 6; BAG family molecular chaperone regulator 6; BAG6; BAG-6; BAT 3; Bat3; BCL2 associated athanogene 6; BCL2-associated athanogene 6; D17H6S52E; D6S52E; DAQB-195H10.3; G3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; large proline-rich protein BAT3; Protein G3; Protein Scythe; Scythe
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Antigen: Scythe/Bat3
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Concentration: 80 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Gene Alias: 2410045D21Rik; AA408914; BAG 6; BAG family molecular chaperone regulator 6; BAG6; BAG-6; BAT 3; Bat3; BCL2 associated athanogene 6; BCL2-associated athanogene 6; D17H6S52E; D6S52E; DAQB-195H10.3; G3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; large proline-rich protein BAT3; Protein G3; Protein Scythe; Scythe
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Antigen:
Scythe/Bat3
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Concentration:
80 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Gene Alias:
2410045D21Rik; AA408914; BAG 6; BAG family molecular chaperone regulator 6; BAG6; BAG-6; BAT 3; Bat3; BCL2 associated athanogene 6; BCL2-associated athanogene 6; D17H6S52E; D6S52E; DAQB-195H10.3; G3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; large proline-rich protein BAT3; Protein G3; Protein Scythe; Scythe
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Antigen: beta Amylase
Applications: ELISA, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary: Primary
Product Type: Antibody
Concentration: 1 mg/mL
Classification: Polyclonal
Form: Lyophilized
Regulatory Status: RUO
Gene Alias: ß-amylase
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Antigen:
beta Amylase
Applications:
ELISA, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Primary or Secondary:
Primary
Product Type:
Antibody
Concentration:
1 mg/mL
Classification:
Polyclonal
Form:
Lyophilized
Regulatory Status:
RUO
Gene Alias:
ß-amylase
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.