Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), eFluor™ 660, eBioscience™, Invitrogen™

Description

• The V3NTY24 MAb recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46
• 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3
• In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation
• Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation
• Studies have identified several kinases that phosphorylate 4E-BP1
• For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111
• Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin
• This gene encodes one member of a family of translation repressor proteins
• The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs
• Interaction of this protein with eIF4E inhibits complex assembly and represses translation
• This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.