beta-3 Tubulin Monoclonal Antibody (2G10-TB3), eBioscience™, Invitrogen™

Description

• Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network
• Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals
• Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone
• Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells
• Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III
• Applications Reported: This 2G10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections, and immunocytochemistry
• Applications Tested: This 2G10 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation
• It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells
• The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.