beta-NGF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human beta-NGF
• Anti-Human beta-NGF-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human beta-NGF (500-P85) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human beta-NGF
• Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human beta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P85BT-1MG will be provided as 2 x 500 μg Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells
• The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites
• Although both types of receptor bind NGF only the high affinity form is capable of signal transduction
• Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex
• Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species
• NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons
• Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons
• Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.