VEGFB Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B
• Anti-Human VEGF-B-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF-B matrix
• Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF-B (500-P267BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B
• Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors
• VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis
• VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF
• There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186
• VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted
• VEGFB stimulates endothelial cell proliferation
• VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2
• Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas
• It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis
• The genes that encode VEGFB have been mapped to chromosome 11q13.