WNT1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-1
• Anti-Human Wnt-1-specific antibody was purified by affinity chromatography employing an immobilized Human Wnt-1 matrix
• Sandwich ELISA: To detect Human Wnt-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Wnt-1 (500-P250Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Wnt-1
• Western Blot: To detect Human Wnt-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Wnt-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal
• The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade
• Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor
• Extensive research has shown that deregulation of these pathways or its components can lead to cancer
• Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form
• At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1
• Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways
• Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis
• Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.