TNFR1 (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I
• Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I
• Western Blot: To detect hsTNF-Receptor I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P143BT-1MG will be provided as 2 x 500 μg TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors
• TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression
• The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1)
• In humans, the TNFR1 gene is located on chromosome 12
• Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor
• Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.