7245589

IFN gamma Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IFN gamma

Applications

ELISA, Western Blot

Conjugate

Unconjugated

Host Species

Rabbit

Target Species

Mouse

Gene Accession No.

P01580

Gene ID (Entrez)

15978

Immunogen

E.coli-derived Recombinant Murine IFN-gamma

Purification Method

Antigen affinity chromatography

Gene

IFNG

Concentration

0.1-1.0 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

PBS with no preservative

Gene Alias

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Gene Symbols

IFNG

Primary or Secondary

Primary

Content And Storage

-20°C

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Description

  • AA Sequence of recombinant protein: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
  • Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IFN-gamma
  • Anti-Murine IFN-gamma-specific antibody was purified by affinity chromatography employing an immobilized Murine IFN-gamma matrix
  • Sandwich ELISA: To detect Murine IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
  • This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IFN-gamma (500-P119Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IFN-gamma
  • Western Blot: To detect Murine IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
  • When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
  • IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
  • IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
  • IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
  • On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
  • The biological activity of the IFN gamma homodimer is highly species specific
  • Human IFN gamma does not show cross-reactivity with mouse
  • IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
  • IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
  • Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
  • IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
  • IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
  • IFN gamma gene mutations are associated with aplastic anemia.

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Show Difference

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Fischer Scientific

7245589

Rabbit Polyclonal Antibody...


Antigen:
IFN gamma

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Mouse

Gene Accession No.:
P01580

Gene ID (Entrez):
15978

Immunogen:
E.coli-derived Recombinant Murine IFN-gamma

Purification Method:
Antigen affinity chromatography

Gene:
IFNG

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Gene Symbols:
IFNG

Primary or Secondary:
Primary

Content And Storage:
-20°C

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Mouse Monoclonal Antibody...


Antigen:
CD123

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 786

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P26951

Gene ID (Entrez):
3563

Immunogen:
__

Purification Method:
Affinity chromatography

Gene:
IL3RA

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD123; CD123 antigen; CDw123; Cyrl; cytokine receptor-like protein CYRL; hIL-3Ra; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; Il3ra; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha; interleukin 3 receptor, alpha (low affinity); interleukin 3 receptor, alpha chain; interleukin-3 receptor class II alpha chain; Interleukin-3 receptor subunit alpha; Sut-1

Gene Symbols:
IL3RA

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Rabbit Polyclonal Antibody...


Antigen:
IL-13

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Rat

Gene Accession No.:
P42203

Gene ID (Entrez):
116553

Immunogen:
E.coli-derived Recombinant Rat IL-13 (113 a.a.)

Purification Method:
Antigen affinity chromatography

Gene:
Il13

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
ALRH; ALRH antibody; BHR1; il 13; Il13; Il-13; IL-13; cytokine; ILN; Interleukin; interleukin 13; Interleukin13; interleukin-13; interleukin-13 precursor; L13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600; P600 antibody; T-cell activation protein P600

Gene Symbols:
Il13

Primary or Secondary:
Primary

Content And Storage:
-20°C

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Mouse Monoclonal Antibody...


Antigen:
CD14

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 786

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P08571

Gene ID (Entrez):
929

Immunogen:
__

Purification Method:
Affinity chromatography

Gene:
Cd14

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14

Gene Symbols:
Cd14

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!