7245604

IFN gamma Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IFN gamma

Applications

ELISA, Western Blot

Conjugate

Biotin

Host Species

Rabbit

Target Species

Human

Gene Accession No.

P01579

Gene ID (Entrez)

3458

Immunogen

E.coli-derived Recombinant Human IFN-gamma

Purification Method

Antigen affinity chromatography

Gene

IFNG

Concentration

0.1-1.0 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

PBS with no preservative

Gene Alias

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Gene Symbols

IFNG

Primary or Secondary

Primary

Content And Storage

-20°C

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Description

  • AA Sequence of recombinant protein: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
  • Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-gamma
  • Anti-Human IFN-gamma-specific antibody was purified by affinity chromatography and then biotinylated
  • Sandwich ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
  • This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IFN-gamma (500-P32) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-gamma
  • Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
  • When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
  • 500-P32BT-1MG will be provided as 2 x 500 μg IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
  • IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
  • IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
  • On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
  • The biological activity of the IFN gamma homodimer is highly species specific
  • Human IFN gamma does not show cross-reactivity with mouse
  • IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
  • IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
  • Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
  • IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
  • IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
  • IFN gamma gene mutations are associated with aplastic anemia.

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Show Difference

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Fischer Scientific

7245604

Rabbit Polyclonal Antibody...


Antigen:
IFN gamma

Applications:
ELISA, Western Blot

Conjugate:
Biotin

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
P01579

Gene ID (Entrez):
3458

Immunogen:
E.coli-derived Recombinant Human IFN-gamma

Purification Method:
Antigen affinity chromatography

Gene:
IFNG

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

Gene Symbols:
IFNG

Primary or Secondary:
Primary

Content And Storage:
-20°C

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7245605

Goat Polyclonal Antibody...


Antigen:
CXCL12 beta (SDF-1 beta)

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Goat

Target Species:
Human

Gene Accession No.:
P48061

Gene ID (Entrez):
6387

Immunogen:
E.coli-derived Recombinant Human SDF-1beta (CXCL12)

Purification Method:
Antigen affinity chromatography

Gene:
Cxcl12

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; CXCL12; Intercrine reduced in hepatomas; IRH; PBSF; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SCYB12; Sdf1; SDF-1; sdf-1 beta; SDF-1 gamma; SDF1A; SDF-1-alpha(3-67); SDF1B; SDF-1-beta(3-72); Stromal cell-derived factor 1; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tpar1

Gene Symbols:
Cxcl12

Primary or Secondary:
Primary

Content And Storage:
-20°C

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7245606

Mouse Monoclonal Antibody...


Antigen:
Glucose Oxidase

Applications:
ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Fungi

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
Purified glucose oxidase from Aspergillus niger.

Purification Method:
Protein G

Gene:
__

Concentration:
1.7 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.4

Gene Alias:
aspergillus niger glucose oxidase; GOx

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles

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7245606

Mouse Monoclonal Antibody...


Antigen:
Glucose Oxidase

Applications:
ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Fungi

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
Purified glucose oxidase from Aspergillus niger.

Purification Method:
Protein G

Gene:
__

Concentration:
1.7 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.4

Gene Alias:
aspergillus niger glucose oxidase; GOx

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles