7245611

IL-10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IL-10

Applications

ELISA, Western Blot

Conjugate

Biotin

Host Species

Rabbit

Target Species

Rat

Gene Accession No.

P29456

Gene ID (Entrez)

25325

Immunogen

E.coli-derived Recombinant Rat IL-10

Purification Method

Antigen affinity chromatography

Gene

IL10

Concentration

0.1-1.0 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

PBS with no preservative

Gene Alias

CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

Gene Symbols

IL10

Primary or Secondary

Primary

Content And Storage

-20°C

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Description

  • AA Sequence of recombinant protein: SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN
  • Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-10
  • Anti-Rat IL-10-specific antibody was purified by affinity chromatography and then biotinylated
  • Sandwich ELISA: To detect Rat IL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
  • This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-10 (500-P139) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-10
  • Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
  • Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
  • 500-P139BT-1MG will be provided as 2 x 500 μg IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
  • This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
  • It also enhances B cell survival, proliferation, and antibody production
  • Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
  • Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
  • Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
  • Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

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Show Difference

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Fischer Scientific

7245611

Rabbit Polyclonal Antibody...


Antigen:
IL-10

Applications:
ELISA, Western Blot

Conjugate:
Biotin

Host Species:
Rabbit

Target Species:
Rat

Gene Accession No.:
P29456

Gene ID (Entrez):
25325

Immunogen:
E.coli-derived Recombinant Rat IL-10

Purification Method:
Antigen affinity chromatography

Gene:
IL10

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

Gene Symbols:
IL10

Primary or Secondary:
Primary

Content And Storage:
-20°C

Img

Fischer Scientific

7245612

Mouse Monoclonal Antibody...


Antigen:
E. coli serotype O

Applications:
ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Bacteria

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
E. coli serotypes: O18, O44, O112, O125.

Purification Method:
Protein A

Gene:
__

Concentration:
0.9 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.4

Gene Alias:
Escherichia coli

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles

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7245612

Mouse Monoclonal Antibody...


Antigen:
E. coli serotype O

Applications:
ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Bacteria

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
E. coli serotypes: O18, O44, O112, O125.

Purification Method:
Protein A

Gene:
__

Concentration:
0.9 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.4

Gene Alias:
Escherichia coli

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles

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7245614

Rabbit Polyclonal Antibody...


Antigen:
CCL5 (RANTES)

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
P13501

Gene ID (Entrez):
6352

Immunogen:
E.coli-derived Recombinant Human RANTES (CCL5)

Purification Method:
Antigen affinity chromatography

Gene:
CCL5

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
beta-chemokine RANTES; CC chemokine; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES; RANTES protein; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; Scya5; SIS d; SISd; SIS-delta; SISd; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; Unknown (protein for MGC:127014)

Gene Symbols:
CCL5

Primary or Secondary:
Primary

Content And Storage:
-20°C