7244988

IL-10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IL-10

Applications

ELISA, Western Blot

Conjugate

Biotin

Host Species

Rabbit

Target Species

Human

Gene Accession No.

P22301

Gene ID (Entrez)

3586

Immunogen

E.coli-derived Recombinant Human IL-10

Purification Method

Antigen affinity chromatography

Gene

IL10

Concentration

0.1-1.0 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

PBS with no preservative

Gene Alias

CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

Gene Symbols

IL10

Primary or Secondary

Primary

Content And Storage

-20°C

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Description

  • AA Sequence of recombinant protein: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
  • Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-10
  • Anti-Human IL-10-specific antibody was purified by affinity chromatography and then biotinylated
  • Sandwich ELISA: To detect hIL-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
  • This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-10 (500-P20) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-10
  • Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
  • Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
  • 500-P20BT-1MG will be provided as 2 x 500 μg IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
  • This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
  • It also enhances B cell survival, proliferation, and antibody production
  • Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
  • Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
  • Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
  • Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

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Fischer Scientific

7244988

Rabbit Polyclonal Antibody...


Antigen:
IL-10

Applications:
ELISA, Western Blot

Conjugate:
Biotin

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
P22301

Gene ID (Entrez):
3586

Immunogen:
E.coli-derived Recombinant Human IL-10

Purification Method:
Antigen affinity chromatography

Gene:
IL10

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

Gene Symbols:
IL10

Primary or Secondary:
Primary

Content And Storage:
-20°C

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7244993

Goat Polyclonal Antibody...


Antigen:
BAFF

Applications:
ELISA, Western Blot

Conjugate:
Biotin

Host Species:
Goat

Target Species:
Human

Gene Accession No.:
Q9Y275

Gene ID (Entrez):
10673

Immunogen:
E.coli-derived Recombinant Human BAFF

Purification Method:
Antigen affinity chromatography

Gene:
TNFSF13B

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
ApoL related ligand TALL-1; b cell-activating factor; B lymphocyte stimulator; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; D8Ertd387e; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; RGD1561519; TALL1; TALL-1; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; Tnfsf13b; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; UNQ401/PRO738; ZTNF4

Gene Symbols:
TNFSF13B

Primary or Secondary:
Primary

Content And Storage:
-20°C

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7244995

Mouse Monoclonal Antibody...


Antigen:
DNA

Applications:
ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Multi-species

Gene Accession No.:
__

Gene ID (Entrez):
__

Immunogen:
Calf thymus DNA.

Purification Method:
PEG precipitation

Gene:
__

Concentration:
1.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.1% sodium azide; pH 7.4

Gene Alias:
dsdna; e. coli dna; single-stranded DNA

Gene Symbols:
__

Primary or Secondary:
Primary

Content And Storage:
4° C, do not freeze

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7244998

Rabbit Polyclonal Antibody...


Antigen:
Neurturin

Applications:
ELISA, Western Blot

Conjugate:
Unconjugated

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
Q99748

Gene ID (Entrez):
4902

Immunogen:
E.coli-derived Recombinant Human Neurturin

Purification Method:
Antigen affinity chromatography

Gene:
NRTN

Concentration:
0.1-1.0 mg/mL

Classification:
Polyclonal

Form:
Lyophilized

Regulatory Status:
RUO

Formulation:
PBS with no preservative

Gene Alias:
neurturin; NRTN; NTN; prepro-neurturin

Gene Symbols:
NRTN

Primary or Secondary:
Primary

Content And Storage:
-20°C