CD197 (CCR7) Monoclonal Antibody (3D12), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2
• This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g
• Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells
• Due to alternative splicing, CD366 exists as membrane-bound and soluble forms
• Galectin-9 has been identified as the ligand for CD366
• In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines
• CD366 has also been implicated in tolerance, autoimmune disease (e.g
• multiple sclerosis), and HIV infection
• Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This F38-2E2 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This may be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
• We recommend using a 780/60 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines
• CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes
• CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine
• CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation
• The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor
• ESTs have been isolated from blood, embryo, lymph node, and thymus libraries
• Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen
• The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.