ABE286025UL
KLF4, Rabbit anti-Mouse, Polyclonal, Millipore Sigma™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE286025UL-Each-of-1 | In Stock | ₹ 16,456.61 |
ABE286025UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
KLF4
Classification
Polyclonal
Dilution
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Gene Alias
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Host Species
Rabbit
Molecular Weight of Antigen
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μL
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_034767
Target Species
Mouse
Form
Serum
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Formulation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Symbols
Klf4;Ezf;Gklf;Zie
Immunogen
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype
IgG
Related Products
Description
- Anti-KLF4, Cat
- No
- ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting
- Krueppel-like factor 4 (UniProt: Q60793; also known as Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4) is encoded by the Klf4 (also known as Ezf, Gklf, Zie) gene (Gene ID: 16600) in murine species
- Klf4 is a member of the krueppel C2H2-type zinc-finger protein family that regulates multiple biological processes, including proliferation, differentiation, development, and apoptosis
- KLF4 is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and can be used as a marker of the pluripotent state for cells in culture
- It can serve both as activator and as repressor depending upon its environment, but its most prominent function is growth suppression
- Interestingly, increased expression of Klf4 leads to an increase in expression of P21WAF1/CIP1, which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries
- Klf4 also mediates the normal functions of the tumor suppressor p53 and contributes to the down-regulation of p53/TP53 transcription
- Mutations in Klf4 gene can lead to unchecked growth and tumor formation
- KLF4 is shown to be often mutated in many colon cancer cell lines and the lack of Klf4 expression is correlated with colorectal cancer pathogenesis
- Elevated expression of Klf4 gene has been linked to gastric cancer, breast cancer, and squamous cell carcinomas
- KLF4 is reported to target the primary miR1-2 loop promoter and stimulates miR-1 expression
- Loss of KLF4 expression is considered as a mechanistic link between aggressive prostate cancer progression and low canonical AR output through miR-1 inactivation
- (Ref.: Jiang, J., et al
- (2008)
- Nat
- Cell Biol
- 10(3); 353-360; Siu, MK et al
- (2016)
- Oncogenesis 5: e282).
Compare Similar Items
Show Difference
Antigen: KLF4
Classification: Polyclonal
Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Gene Alias: Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Host Species: Rabbit
Molecular Weight of Antigen: ∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μL
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): NP_034767
Target Species: Mouse
Form: Serum
Applications: ChIP Assay, Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Symbols: Klf4;Ezf;Gklf;Zie
Immunogen: GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG
Antigen:
KLF4
Classification:
Polyclonal
Dilution:
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Gene Alias:
Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
Host Species:
Rabbit
Molecular Weight of Antigen:
∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μL
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
NP_034767
Target Species:
Mouse
Form:
Serum
Applications:
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Gene Symbols:
Klf4;Ezf;Gklf;Zie
Immunogen:
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG
Antigen: E3 ubiquitin-protein ligase (UBR5/EDD)
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 25 μL
Research Discipline: Epigenetics and Nuclear Function
Gene ID (Entrez): __
Target Species: Mouse, Human
Form: Serum
Applications: Western Blot, Immunofluorescence, Immunoprecipitation
Conjugate: Unconjugated
Formulation: __
Gene Symbols: UBR5,EDD,EDD1,HYD,KIAA0896
Immunogen: His-tagged recombinant fragment corresponding to 331 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR5/EDD.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase UBR5/EDD in human and murine cells. It targets an epitope within 331 amino acids from the internal region.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG
Antigen:
E3 ubiquitin-protein ligase (UBR5/EDD)
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
25 μL
Research Discipline:
Epigenetics and Nuclear Function
Gene ID (Entrez):
__
Target Species:
Mouse, Human
Form:
Serum
Applications:
Western Blot, Immunofluorescence, Immunoprecipitation
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
UBR5,EDD,EDD1,HYD,KIAA0896
Immunogen:
His-tagged recombinant fragment corresponding to 331 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR5/EDD.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase UBR5/EDD in human and murine cells. It targets an epitope within 331 amino acids from the internal region.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG
Antigen: __
Classification: __
Dilution: __
Gene Alias: __
Host Species: __
Molecular Weight of Antigen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
Applications: __
Conjugate: __
Formulation: __
Gene Symbols: __
Immunogen: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Antigen:
__
Classification:
__
Dilution:
__
Gene Alias:
__
Host Species:
__
Molecular Weight of Antigen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__
Form:
__
Applications:
__
Conjugate:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Antigen: Meis1
Classification: Polyclonal
Dilution: Western Blotting Analysis: 1μg/mL of this antibody detected Meis1 in 10μg of K562 cell lysate. Immunofluorescence Analysis: A representative lot detected Meis1 in adjacent stage (St) 41 sagittal hindbrain and eye sections (Mercader, N., et. al. (2005). Development. 132(18):4131-42). Western Blotting Analysis: A 1:800 dilution from a representative lot detected Meis1 in HEK293T cells transfectd with Meis1a (C ourtesy of Dr. Valeria Azcoitia and Dr. Miguel Torres ). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33). Western Blotting Analysis: A representative lot detected Meis1 in proximal, distal, and RA-treated distal blastemas (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
Gene Alias: Homebox protein Meis1
Host Species: Rabbit
Molecular Weight of Antigen: 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Gene ID (Entrez): __
Target Species: Human, Mouse
Form: Purified
Applications: ChIP Assay, Immunofluorescence, Western Blot
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
Gene Symbols: Meis1
Immunogen: KLH-conjugated linear peptide corresponding 14 amino acids from the C-terminal region of Meis1.
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This rabbit polyclonal antibody detects Meis1 in human and murine cells. It targets an epitope within the C-terminal region.
Content And Storage: Stable for 1 year at -20°C from date of receipt.
Isotype: IgG
Antigen:
Meis1
Classification:
Polyclonal
Dilution:
Western Blotting Analysis: 1μg/mL of this antibody detected Meis1 in 10μg of K562 cell lysate. Immunofluorescence Analysis: A representative lot detected Meis1 in adjacent stage (St) 41 sagittal hindbrain and eye sections (Mercader, N., et. al. (2005). Development. 132(18):4131-42). Western Blotting Analysis: A 1:800 dilution from a representative lot detected Meis1 in HEK293T cells transfectd with Meis1a (C ourtesy of Dr. Valeria Azcoitia and Dr. Miguel Torres ). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1 in Chromatin Immunoprecipitation applications (Penkov, D., et. al. (2013). Cell Rep. 3(4):1321-33). Western Blotting Analysis: A representative lot detected Meis1 in proximal, distal, and RA-treated distal blastemas (Mercader, N., et. al. (2005). Development. 132(18):4131-42).
Gene Alias:
Homebox protein Meis1
Host Species:
Rabbit
Molecular Weight of Antigen:
52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μg
Research Discipline:
Epigenetics & Nuclear Function
Gene ID (Entrez):
__
Target Species:
Human, Mouse
Form:
Purified
Applications:
ChIP Assay, Immunofluorescence, Western Blot
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
Gene Symbols:
Meis1
Immunogen:
KLH-conjugated linear peptide corresponding 14 amino acids from the C-terminal region of Meis1.
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This rabbit polyclonal antibody detects Meis1 in human and murine cells. It targets an epitope within the C-terminal region.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.
Isotype:
IgG