ABS220025UL

FtsZ (GTPase), Rabbit anti-Bacteria, Polyclonal, Millipore Sigma™

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 ABS220025UL-Each-of-1 In Stock ₹ 16,456.61

ABS220025UL - Each of 1

₹ 16,456.61

In Stock

Quantity

1

Base Price: ₹ 16,456.61

GST (18%): ₹ 2,962.19

Total Price: ₹ 19,418.80

Antigen

FtsZ (GTPase)

Classification

Polyclonal

Dilution

Western Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).

Gene Alias

Cell division protein FtsZ;Cell Division FtsZ GTPase

Host Species

Rabbit

Molecular Weight of Antigen

∽44 kDa observed; 40.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Quantity

25 μL

Research Discipline

Signaling

Gene ID (Entrez)

NP_389412

Target Species

Bacteria

Form

Serum

Applications

Immunofluorescence, Western Blot

Conjugate

Unconjugated

Formulation

Rabbit polyclonal antiserum with 0.05% sodium azide.

Gene Symbols

FtsZ;BSU15290

Immunogen

Full length purified FtsZ from Bacillus subtilis.

Purification Method

Unpurified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.

Content And Storage

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype

IgG

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Description

  • Anti-FtsZ (GTPase), Cat
  • No
  • ABS2200, is a rabbit polyclonal antibody that detects Cell division protein FtsZ and has been tested for use in Immunofluorescence and Western Blotting
  • Cell division protein FtsZ (UniProt: P17865; also known as Cell Division FtsZ GTPase, FtsZ) is encoded by the FtsZ (also known as BSU15290) gene (Gene ID: 935971) in Bacillus subtilis
  • FtsZ is homodimeric prokaryotic homologue of tubulin with GTPase activity
  • It is found in nearly all bacteria
  • It polymerizes in the presence of GTP to form higher-ordered polymers
  • It contains three GTP-binding sites that are located in the N-terminal region
  • The less conserved C-terminal domain contains residues that are important for GTP hydrolysis
  • FtsZ is shown to be essential for bacterial cell division and in association with FtzA (FtsAZ) it forms a contractile ring structure (Z ring) at the site of cell division
  • The regulation of the ring assembly controls the timing and the location of cell division
  • One of the major functions of FtsZ is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells
  • The FtsZ treadmilling rate controls the rate of peptidoglycan synthesis and cell division
  • During bacterial cell division FtsZ co-localizes with SpoIIE, a dual function protein that contributes to the switch form medial to polar cell division during sporulation
  • The anti-FtsZ blot has also been used as control for sample loading
  • (Ref.: Bisson-Filho, AW., et al., (2017)
  • Science 355(6326); 739-743; Feucht, A., and Jeffery Errington, J (2005)
  • Microbiology 151(6); 2053-2064; Lucet, I., et al
  • (2000)
  • EMBO J
  • 19(7); 1467-1475)
  • Lytvynenko, I., et al
  • (2019)
  • Cell
  • 178(1); 76-90)

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Fischer Scientific

ABS220025UL

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Antigen:
FtsZ (GTPase)

Classification:
Polyclonal

Dilution:
Western Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).

Gene Alias:
Cell division protein FtsZ;Cell Division FtsZ GTPase

Host Species:
Rabbit

Molecular Weight of Antigen:
∽44 kDa observed; 40.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Quantity:
25 μL

Research Discipline:
Signaling

Gene ID (Entrez):
NP_389412

Target Species:
Bacteria

Form:
Serum

Applications:
Immunofluorescence, Western Blot

Conjugate:
Unconjugated

Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.

Gene Symbols:
FtsZ;BSU15290

Immunogen:
Full length purified FtsZ from Bacillus subtilis.

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.

Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype:
IgG

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Antigen:
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Classification:
__

Dilution:
__

Gene Alias:
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Host Species:
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Molecular Weight of Antigen:
__

Quantity:
__

Research Discipline:
__

Gene ID (Entrez):
__

Target Species:
__

Form:
__

Applications:
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Conjugate:
__

Formulation:
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Immunogen:
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Purification Method:
__

Regulatory Status:
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__

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__

Isotype:
__

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Antigen:
UBE2A

Classification:
Polyclonal

Dilution:
Western Blotting Analysis: A 1:500 dilution from a representative lot detected UBE2A in mouse kidney tissue lysate.Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE2A in human pancreas, human cervical cancer, and human uterus tissues.

Gene Alias:
Ubiquitin-conjugating enzyme E2A;EC 2.3.2.23;E2 ubiquitin-conjugating enzyme A;RAD6 homolog A;HR6A;hHR6A;Ubiquitin carrier protein A;Ubiquitin-protein ligase A

Host Species:
Rabbit

Molecular Weight of Antigen:
∽17 kDa observed; 17.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Quantity:
25 μg

Research Discipline:
Signaling

Gene ID (Entrez):
__

Target Species:
Human, Mouse

Form:
__

Applications:
Immunohistochemistry (Paraffin), Western Blot

Conjugate:
Unconjugated

Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols:
UBE2A;RAD6A

Immunogen:
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Ubiquitin-conjugating enzyme E2 A (UBE2A).

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This rabbit polyclonal antibody detects Ubiquitin-conjugating enzyme E2 A (UBE2A) in HeLa cells and Mouse Kidney.

Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.

Isotype:
__

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