ABS210725UL
phospho-CaMKII beta (Ser331), Rabbit anti-Rat, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS210725UL-Each-of-1 | In Stock | ₹ 16,456.61 |
ABS210725UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
phospho-CaMKII beta (Ser331)
Classification
Polyclonal
Dilution
Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Gene Alias
Calcium/calmodulin-dependent protein kinase type II subunit beta;EC: 2.7.11.17;CaM kinase II subunit beta;CaMK-II beta
Host Species
Rabbit
Molecular Weight of Antigen
∽60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μL
Research Discipline
Neuroscience
Test Specificity
This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Applications
Immunocytochemistry, Western Blot
Conjugate
Unconjugated
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Camk2b
Immunogen
A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Rat
Isotype
IgG
Related Products
Description
- Anti-phospho-CaMKII beta (Ser331), Cat
- No
- ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in immunocytochemistry and Western Blotting
- Calcium/calmodulin-dependent protein kinase type II subunit beta (UniProt: P08413; also known as EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II subunit beta) is encoded by the Camk2b gene (Gene ID: 24245) in rat
- CaM kinase II is one of the most widely studied multi-functional enzyme
- It is found in high concentrations in the brain and in some regions it may constitute up to 2% of the total protein content
- CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (alpha, beta, gamma, and delta)
- CaMKII beta contains an auto-inhibitory domain (aa 283-293), a calmodulin-binding domain (aa 291-301) and a unique C-terminal association domain responsible for oligomerization
- It is activated Ca2+/calmodulin and binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-287 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity that persists even after withdrawal of calcium
- Phosphorylation of Thr-287 locks the kinase into an activated state
- CaM Kinase II typically recognizes the phosphorylation site sequence Arg-X-X-Ser/Thr
- It prefers arginine in the P3 position, which is reported to make an electrostatic contact with Glu96 in the catalytic core.
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Antigen: phospho-CaMKII beta (Ser331)
Classification: Polyclonal
Dilution: Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Gene Alias: Calcium/calmodulin-dependent protein kinase type II subunit beta;EC: 2.7.11.17;CaM kinase II subunit beta;CaMK-II beta
Host Species: Rabbit
Molecular Weight of Antigen: ∽60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μL
Research Discipline: Neuroscience
Test Specificity: This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Applications: Immunocytochemistry, Western Blot
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Camk2b
Immunogen: A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Rat
Isotype: IgG
Antigen:
phospho-CaMKII beta (Ser331)
Classification:
Polyclonal
Dilution:
Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Gene Alias:
Calcium/calmodulin-dependent protein kinase type II subunit beta;EC: 2.7.11.17;CaM kinase II subunit beta;CaMK-II beta
Host Species:
Rabbit
Molecular Weight of Antigen:
∽60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μL
Research Discipline:
Neuroscience
Test Specificity:
This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Applications:
Immunocytochemistry, Western Blot
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Camk2b
Immunogen:
A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Rat
Isotype:
IgG
Antigen: __
Classification: __
Dilution: __
Gene Alias: __
Host Species: __
Molecular Weight of Antigen: __
Quantity: __
Research Discipline: __
Test Specificity: __
Content And Storage: __
Applications: __
Conjugate: __
Formulation: __
Gene Symbols: __
Immunogen: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Isotype: __
Antigen:
__
Classification:
__
Dilution:
__
Gene Alias:
__
Host Species:
__
Molecular Weight of Antigen:
__
Quantity:
__
Research Discipline:
__
Test Specificity:
__
Content And Storage:
__
Applications:
__
Conjugate:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Isotype:
__
Antigen: Vaccinia related kinase-1
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 25 μg
Research Discipline: Signaling
Test Specificity: This rabbit polyclonal antibody detects Serine/threonine-protein kinase VRK1 in human cells. It targets an epitope within 30 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: ELISA, Western Blot
Conjugate: Unconjugated
Formulation: __
Gene Symbols: VRK1
Immunogen: KLH-conjugated linear peptide corresponding to 30 amino acids from the N-terminal region of human Vaccinia-related kinase 1 (VRK1).
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Isotype: IgG
Antigen:
Vaccinia related kinase-1
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
25 μg
Research Discipline:
Signaling
Test Specificity:
This rabbit polyclonal antibody detects Serine/threonine-protein kinase VRK1 in human cells. It targets an epitope within 30 amino acids from the N-terminal region.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
ELISA, Western Blot
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
VRK1
Immunogen:
KLH-conjugated linear peptide corresponding to 30 amino acids from the N-terminal region of human Vaccinia-related kinase 1 (VRK1).
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
IgG
Antigen: Vaccinia related kinase-1
Classification: Polyclonal
Dilution: __
Gene Alias: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 25 μg
Research Discipline: Signaling
Test Specificity: This rabbit polyclonal antibody detects Serine/threonine-protein kinase VRK1 in human cells. It targets an epitope within 128 amino acids from the C-terminal region.
Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: ChIP Assay
Conjugate: Unconjugated
Formulation: __
Gene Symbols: VRK1
Immunogen: His-tagged recombinant fragment corresponding to 128 amino acids from the C-terminal region of human Vaccinia-related kinase-1 (VRK1).
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Isotype: IgG
Antigen:
Vaccinia related kinase-1
Classification:
Polyclonal
Dilution:
__
Gene Alias:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
25 μg
Research Discipline:
Signaling
Test Specificity:
This rabbit polyclonal antibody detects Serine/threonine-protein kinase VRK1 in human cells. It targets an epitope within 128 amino acids from the C-terminal region.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
ChIP Assay
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
VRK1
Immunogen:
His-tagged recombinant fragment corresponding to 128 amino acids from the C-terminal region of human Vaccinia-related kinase-1 (VRK1).
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
IgG