MABT84125UG
Podoplanin, Mouse anti-Monkey, Human, Clone: LpMab-12, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT84125UG-Each-of-1 | In Stock | ₹ 16,456.61 |
MABT84125UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Podoplanin
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
PDPN;GP36;PSEC0003;PSEC0025
Immunogen
LN229 glioma cells expressing human Podoplanin.
Quantity
25 μg
Research Discipline
Cell Structure
Test Specificity
Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone
LpMab-12
Dilution
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
Gene Alias
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Monkey, Human
Form
Purified
Related Products
Description
- Anti-Podoplanin, clone LpMab-12, Cat
- No
- MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin)
- Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human
- Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes
- It may be involved in cell migration and/or actin cytoskeleton organization
- Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles
- Six isoforms of podoplanin have been described that are produced by alternative splicing
- It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form
- Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162)
- Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus
- Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2)
- Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3
- Clone LpMab-12 specifically detects sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin
- It also reacts with the synthetic glycopeptide of hPDPN, corresponding to 38 54 amino acids that carries 2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52
- The minimal epitope of LpMab-12 is identified as Asp49 Pro53 of hPDPN
- (Ref.: Kato, Y et al
- (2016)
- PLoS ONE 11(3): e0152912).
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Antigen: Podoplanin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: PDPN;GP36;PSEC0003;PSEC0025
Immunogen: LN229 glioma cells expressing human Podoplanin.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone: LpMab-12
Dilution: Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Monkey, Human
Form: Purified
Antigen:
Podoplanin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
PDPN;GP36;PSEC0003;PSEC0025
Immunogen:
LN229 glioma cells expressing human Podoplanin.
Quantity:
25 μg
Research Discipline:
Cell Structure
Test Specificity:
Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone:
LpMab-12
Dilution:
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
Gene Alias:
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Monkey, Human
Form:
Purified
Antigen: __
Classification: __
Conjugate: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Dilution: __
Gene Alias: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Antigen:
__
Classification:
__
Conjugate:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Dilution:
__
Gene Alias:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Antigen: SYNE1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols: __
Immunogen: GST-tagged recombinant human SYNE1 isoform Beta C-terminal 121-a.a. fragment fused to full-length isoform Alpha sequence (UniProt Q8NF91-2 andQ8NF91-3).
Quantity: 100 μg
Research Discipline: Cell Structure
Test Specificity: Epitope region is present in, human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa), mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and rat Nesprin-1 (UniProt Q8VHJ9), but absent from human spliced isoforms 6/7/10 (drop1)/12 (GSRP-56; 56 kDa), mouse spliced isoform 5 (Enaptin-165), or rat spliced isoform CPG2 (UniProt Q63128) reported by UniProt (Q8NF91, Q6ZWR6, Q8VHJ9, Q63128). Clone 7A12 does not reacts with nesprin-2, but stained centrosome in acetone/methanol-fixed HeLa cells due to uncharacterized cross-reactivity (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Applications: Western Blot
Clone: 7A12
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
SYNE1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Symbols:
__
Immunogen:
GST-tagged recombinant human SYNE1 isoform Beta C-terminal 121-a.a. fragment fused to full-length isoform Alpha sequence (UniProt Q8NF91-2 andQ8NF91-3).
Quantity:
100 μg
Research Discipline:
Cell Structure
Test Specificity:
Epitope region is present in, human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa), mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and rat Nesprin-1 (UniProt Q8VHJ9), but absent from human spliced isoforms 6/7/10 (drop1)/12 (GSRP-56; 56 kDa), mouse spliced isoform 5 (Enaptin-165), or rat spliced isoform CPG2 (UniProt Q63128) reported by UniProt (Q8NF91, Q6ZWR6, Q8VHJ9, Q63128). Clone 7A12 does not reacts with nesprin-2, but stained centrosome in acetone/methanol-fixed HeLa cells due to uncharacterized cross-reactivity (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Western Blot
Clone:
7A12
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: MYH-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: Myh1
Immunogen: KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-1.
Quantity: 100 μg
Research Discipline: Cell Structure
Test Specificity: Clone 6F12H3 is a rat monoclonal antibody that detects Myosin-1 in mouse and rat muscles. It target an epitope within the coiled coil domain in the C-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Clone: 6F12H3
Dilution: __
Gene Alias: __
Host Species: Rat
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse, Rat
Form: Purified
Antigen:
MYH-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
Myh1
Immunogen:
KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of murine Myosin-1.
Quantity:
100 μg
Research Discipline:
Cell Structure
Test Specificity:
Clone 6F12H3 is a rat monoclonal antibody that detects Myosin-1 in mouse and rat muscles. It target an epitope within the coiled coil domain in the C-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Immunofluorescence, Immunohistochemistry, Western Blot
Clone:
6F12H3
Dilution:
__
Gene Alias:
__
Host Species:
Rat
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Mouse, Rat
Form:
Purified