AP106450UG
MilliporeSigma™ anti-PhosphoDetect PDH-E1alpha (pSer300), Polyclonal
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AP106450UG-Each-of-1 | In Stock | ₹ 47,071.69 |
AP106450UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,071.69
GST (18%): ₹ 8,472.904
Total Price: ₹ 55,544.594
Antigen
PhosphoDetect™ PDH-E1α (pSer300)
Classification
Polyclonal
Formulation
In 150mM NaCl, 100mM Tris-Glycine, pH 7.4.
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser300phosphorylation site of human PDH-E1α
Regulatory Status
RUO
Test Specificity
Recognizes the ∽44kDa PDH-E1α protein phosphorylated at Ser300in HEK293 cells.
Content And Storage
−20°C
Applications
Immunocytochemistry
Conjugate
Unconjugated
Host Species
Rabbit
Quantity
50 μg
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Related Products
Description
- Specifically detects PhosphoDetect™ PDH-E1α (pSer 300 ) Clone: in Human samples, and it is validated for Immunocytochemistry The pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle
- Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases
- The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP])
- The activity of the PDC is mainly regulated by phosphorylation-dephosphorylation of three specific serine residues (232, 293, 300) on the E1α subunit
- Phosphorylation of PDH determines the activity of the complex
- Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex
- The activity of the PDP1 isoform is sensitive to Ca 2+ , while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate
- (DCA).
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Antigen: PhosphoDetect™ PDH-E1α (pSer300)
Classification: Polyclonal
Formulation: In 150mM NaCl, 100mM Tris-Glycine, pH 7.4.
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser300phosphorylation site of human PDH-E1α
Regulatory Status: RUO
Test Specificity: Recognizes the ∽44kDa PDH-E1α protein phosphorylated at Ser300in HEK293 cells.
Content And Storage: −20°C
Applications: Immunocytochemistry
Conjugate: Unconjugated
Host Species: Rabbit
Quantity: 50 μg
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
PhosphoDetect™ PDH-E1α (pSer300)
Classification:
Polyclonal
Formulation:
In 150mM NaCl, 100mM Tris-Glycine, pH 7.4.
Immunogen:
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser300phosphorylation site of human PDH-E1α
Regulatory Status:
RUO
Test Specificity:
Recognizes the ∽44kDa PDH-E1α protein phosphorylated at Ser300in HEK293 cells.
Content And Storage:
−20°C
Applications:
Immunocytochemistry
Conjugate:
Unconjugated
Host Species:
Rabbit
Quantity:
50 μg
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
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