CD253 Rat anti-Mouse, Unlabeled, Clone: N2B2, BD

Description

• Description The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40-kDa type-II transmembrane protein of the TNF family
• This antibody recognizes mouse Trail -transfected 2PK-3 cells, but not the parental cell line
• TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells
• Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far
• The pattern of expression of mouse TRAIL is controversial
• TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines
• Similar results were obtained by western blot and mRNA analyses
• However, these results could not be reproduced with the N2B2 mAb
• TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15
• However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface
• Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb
• TRAIL has been renamed as CD253 recently.