Myogenin Mouse, Unlabeled, Clone: F5D, BD

Description

• Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculin or Myf-6)
• MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes
• The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif
• MyoD family members share about 80% amino acid homology in their bHLH motifs
• Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes
• In addition to activating muscle-specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members
• For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of the endogenous myogenin gene as well as transactivation of MyoD
• Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin
• Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation
• Myogenin migrates at a molecular weight of ∽34kDa by SDS-PAGE
• Clone F5D recognizes human, mouse, rat and cat myogenin
• Mapping studies suggest that F5D reacts with an epitope between amino acids 138-158 of myogenin
• A recombinant fusion protein corresponding to amino acids 30- 224 of rat myogenin was used as immunogen
• Host Species: Mouse Clone: F5D Isotype: IgG1 Species Reactivity: Human Immunogen: Recombinant Rat Myogenin aa
• 30-224 Immunohistochemistry, Immunohistochemistry (Frozen), Western Blotting