p21 Mouse, Unlabeled, Clone: SXM30, BD

Description

• Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes
• Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle
• The activity of these complexes is modulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including, p16, p21, p27, and others
• These proteins, referred to as inhibitors of Cdk activity (CdkIs), bind to cyclins, cdks or their complexes
• p21, also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), and p53-regulated inhibitor of Cdks (Pic1) inhibits cyclin D-cdk4, cyclin E-cdk3, cyclin A-cdk2, and cyclin A-cdk1
• p21 can also inhibit cell cycle progression by binding to PCNA and blocking DNA replication
• p21 has also been shown to be a component of active cyclin-cdk complexes, suggesting that p21-containing complexes may shift between active and inactive states through changes in p21 content
• Active, p21-containing complexes appear to contain one p21 molecule, whereas inactive compexes contain multiple p21 molecules
• The expression of p21 can be induced in response to number of signals, including transcriptional upregulation by the tumor suppressor protein, p53
• Human p21 has a calculated molecular weight of 18kDa and runs at 21kDa in SDS-PAGE
• Clone SXM30 reacts with human, rat, and mouse p21
• With regard to cross-species recognition, SXM30 has similar properties to a related p21 clone, SX118
• Purified, recombinant human p21-fusion protein was used as immunogen
• Host Species: Mouse Clone: SXM30 Isotype: IgG1 κ Species Reactivity: Human, Mouse, Rat Immunogen: Human p21 Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting