Cytochrome c Mouse, Unlabeled, Clone: 7H8.2C12, BD

Description

• A cytochrome is an electron-transporting protein that contains a heme prosthetic group
• Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925
• The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation
• Cytochromes are classified into four groups (a, b, c, and d) according to spectrochemical characteristics, and there are five cytochromes between Coenzyme QH2 and O2 in the electron transport chain
• Cytochrome c is a water-soluble peripheral membrane protein of the mitochondria and transports electrons from the Coenzyme QH2 - Cytochrome c reductase complex to the Cytochrome c oxidase complex
• Recently it has been suggested that Cytochrome c may be required for cells to undergo apoptosis
• Mitochondria may be involved in apoptosis by releasing Cytochrome c to the cytosol where it functions in the activation of Caspase 3 (CPP32), an ICE family molecule that has been shown to trigger apoptosis when activated
• In the cytoplasm Cytochrome C might exist in polymeric forms, appearing as a 58-60kDa band by western blot analysis, rather than the monomeric form
• Monomeric Cytochrome c migrates at a reduced molecular weight of 15kDa
• Clone 7H8.2C12 recognizes the denatured form of pigeon, horse, rat, mouse and human Cytochrome c
• It does not recognize the native form of Cytochrome c
• Clone 7H8.2C12 recognizes an epitope within amino acids 93-104 (inclusive) of pigeon Cytochrome C, based on competitive ELISA results
• Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon Cytochrome c were used as immunogens
• Clone 7H8.2C12 is used for western blot detection of Cytochrome c
• Clone 6H2.B4, Mfr
• No
• 556432, is used for immunoprecipitation
• Cell lines P388D1 mouse lymphoma (ATCC CCL-46), HeLa human carcinoma (ATCC CCL-2), Jurkat T leukemia (ATCC TIB-152), and NIH/3T3 mouse fibroblast cells (ATCC CRL-1658) may be used as positive control cell lysates
• This antibody is routinely tested by western blot analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature
• Host Species: Mouse Clone: 7H8.2C12 Isotype: IgG2b, κ Species Reactivity: Human Immunogen: Synthetic Peptides of Pigeon Cytochrome C Western Blotting